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Flow cytometer and microscope image cells

机译:流式细胞仪和显微镜图像池

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When a marine vessel enters port it may hold water from another part of the world, which might contain foreign organisms such as red tide-a microscopic algae Karenia brevis that produces a toxin that paralyzes fish so they cannot breath. To properly analyze water samples, many research facilities and laboratories use cy-tometers to measure the physical or chemical characteristics of any cells or particles in a fluid sample. Using flow cytometry allows researchers to make measurements as the cells or particles pass through the measuring apparatus. In some systems, using electrical or mechanical methods to divert and collect cells with specific size and/or charge can extend this capability. While doing research on how to correct lens aberrations at the Thayer School of Engineering at Dartmouth College (Hanover, NH, USA), Christian Sieracki developed a depth-of-field (DOF) enhancer: a phased array that elongates the point-spread function (PSF) of light into a narrow pencil of light with a larger DOF. After joining The Bigelow Laboratory for Ocean Sciences (West Boothbay Harbor, ME, USA) in 1997, Sieracki recognized the potential of the device in flow cytometry. With a grant from the National Science Foundation (Arlington, VA, USA), Sieracki developed a more-robust version of the flow cytometer and left to found Fluid Imaging Technologies (Edgecomb, ME, USA) to commercially develop a product now known as the Flow Cytometer and Microscope (FlowCAM; see Fig. 1).
机译:当一艘船舶进入港口时,它可能会容纳来自世界其他地方的水,其中可能含有诸如赤潮之类的外来生物。赤潮是一种微小的藻类短小海藻,能产生使鱼瘫痪的毒素,因此它们无法呼吸。为了正确地分析水样品,许多研究机构和实验室都使用细胞仪来测量液体样品中任何细胞或颗粒的物理或化学特性。使用流式细胞仪可使研究人员在细胞或颗粒通过测量仪器时进行测量。在某些系统中,使用电气或机械方法转移和收集具有特定大小和/或电荷的细胞可以扩展此功能。在达特茅斯学院(美国新罕布什尔州汉诺威)的Thayer工程学院研究如何校正镜头像差时,Christian Sieracki开发了景深(DOF)增强器:一种相控阵,可延长点扩展功能(PSF)的光变成具有较大DOF的窄光铅笔。在1997年加入Bigelow海洋科学实验室(美国缅因州西布斯贝港)后,Sieracki意识到了该设备在流式细胞仪中的潜力。 Sieracki在国家科学基金会(美国弗吉尼亚州阿灵顿)的资助下,开发了更强大的流式细胞仪版本,然后成立了Fluid Imaging Technologies(美国Edgecomb,ME)进行商业开发,该产品现在被称为流式细胞仪和显微镜(FlowCAM;见图1)。

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