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首页> 外文期刊>Journal of Virology >Metabolic Requirements for the Development of Hemadsorption Activity and Virus Formation in BHK-21 Cells Infected with Newcastle Disease Virus
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Metabolic Requirements for the Development of Hemadsorption Activity and Virus Formation in BHK-21 Cells Infected with Newcastle Disease Virus

机译：BHK-21细胞中血液吸附活性和病毒形成发育的代谢要求，感染新城疫病毒病毒

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Differential effect of various metabolic inhibitors on the development of hemadsorption activity and virus formation in cells infected with Newcastle disease virus (NDV) was investigated. It was found that, in BHK-21 cells infected with NDV, cycloheximide did not prevent the development of hemadsorption activity, whereas protein synthesis and virus formation by the cell were rapidly inhibited by the drug. When the drug was added to the culture at 4.5 h after infection or later, hemadsorption activity of the cell continued to develop normally for about 1 h. Similar increase in hemadsorption activity was found in cells which were treated with anti-NDV serum (to neutralize their hemadsorption activity) and then washed and incubated with cycloheximide. However, when cells were treated with the drug early in the infection (1.5 or 3.0 h), they did not show any detectable hemadsorption reaction throughout the infection. In contrast to cycloheximide, iodoacetate added to the culture together with sodium azide inhibited completely both the development of hemadsorption activity and the formation of progeny virus. These results suggest that the change of cell surface to become hemadsorptive may depend upon the energy generating system but not upon de novo synthesis of protein, whereas production of infectious virus may require continuous synthesis of protein.
机译：研究了各种代谢抑制剂对感染新城疫病毒（NDV）感染血液吸附活性和病毒形成的差异效果。结果发现，在感染NDV的BHK-21细胞中，环己酰亚胺并未阻止血液吸附活性的发展，而药物的蛋白质合成和细胞的病毒形成迅速抑制。当感染后4.5小时或后来将药物添加到培养物中时，细胞的血液吸附活性继续正常形成约1小时。在用抗NDV血清处理的细胞中发现血液吸附活性的类似增加（以中和它们的血液吸附活性），然后用环己酰亚胺洗涤并温育。然而，当在感染的早期用药物处理细胞（1.5或3.0h）时，它们在整个感染中没有显示出任何可检测的血液吸附反应。与环己酰亚胺相比，将碘乙酸盐与叠氮酸钠一起添加血液吸附活性和后代病毒的形成。这些结果表明，细胞表面变化变为血液吸收可能取决于能量产生系统，但不适用于蛋白质的Novo合成，而传染性病毒的产生可能需要连续合成蛋白质。

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    《Journal of Virology》 |1973年第4期|共8页
  • 作者

    Yoshiyuki Nagai;

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  • 收录信息 美国《科学引文索引》(SCI);
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