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Translation of Virus mRNA: Synthesis of Bacteriophage Qβ Proteins in a Cell-Free Extract from Wheat Embryo

机译:病毒mRNA的翻译:来自小麦胚胎的无细胞提取物中的噬菌体Qβ蛋白的合成

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The RNA from bacteriophage Qβ can be translated by cell-free extracts from wheat embryos. This translation, by 80S ribosomes, occurs at a low magnesium ion concentration. Three products are synthesized which coelectrophorese with Qβ proteins synthesized in Escherichia coli extracts. The smallest of these has been identified as coat protein. Although the polycistronic bacteriophage message is translated with fidelity, the efficiency is much less than when the monocistronic brome mosaic virus coat protein message is translated.
机译:来自噬菌体Qβ的RNA可以通过小麦胚胎的无细胞提取物翻译。该翻译,80 S 核糖体发生在低镁离子浓度下。合成了三种产品,其中在大肠杆菌中合成的Qβ蛋白质相互作用。这些最小的这些已被鉴定为Coat蛋白。虽然多排态噬菌体消息被忠诚翻译,但效率远低于单闭合斑马镶嵌病毒外套蛋白质消息的效率远低于翻译。

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