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首页> 外文期刊>Journal of Virology >Separation of Herpes Simplex Virus-Induced Antigens by Concanavalin A Affinity Chromatography
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Separation of Herpes Simplex Virus-Induced Antigens by Concanavalin A Affinity Chromatography

机译：通过粗碱素素分离单纯疱疹病毒诱导的抗原A亲和层析

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摘要

Biologically active herpes simplex virus (HSV)-induced antigens were selectively removed from extracts of infected BHK cells by affinity chromatography by utilizing an insoluble form of concanavalin A (Con A). Soluble extracts of 3H-glucosamine-labeled, HSV-infected cells were absorbed to a Con A column. Bound material was eluted with α-methyl-d-mannoside (αMM) and NaCl. The specific activity of the eluted glycoproteins increased by 10-fold. Two broad groups of viral-induced antigens were isolated from Con A. Group I includes two antigens which bind to Con A by a specific mechanism because the antigens are dissociated by αMM. Group II contains three antigens which bind to Con A but apparently by a nonspecific or electrolytic mechanism. One antigen in group I was identified as the glycoprotein antigen, CP-1, described previously.
机译：通过使用不溶性形式的令人溶性的令人溶性形式的令人溶性形式的令人溶性的令人溶性的令人溶性的令人溶性的令人溶性的令人溶性的令人溶性的康昔单蛋白A（CON A），选择性地从感染的BHK细胞提取物中选择性地除去生物活性疱疹病毒（HSV）诱导的抗原。溶于 3 h-glucosamine标记的HSV感染细胞的溶性提取物被吸收到柱上。用α-甲基-D-甘露糖苷（αmm）和NaCl洗脱结合的材料。洗脱的糖蛋白的比活性增加了10倍。从CON A中分离了两组宽的病毒诱导的抗原。基团I包括两种抗原，其结合到特定机制，因为抗原由αmm解离。 II族含有三种抗原，其结合不特异性或电解机制而明显。将一个抗原在Ⅰ组中鉴定为先前描述的糖蛋白抗原CP-1。

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