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首页> 外文期刊>Journal of Virology >Synthesis of the Unusual DNA of Bacillus subtilis Bacteriophage SP-15
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Synthesis of the Unusual DNA of Bacillus subtilis Bacteriophage SP-15

机译：枯草芽孢杆菌菌血管菌的不寻常DNA合成SP-15

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Cultures of Bacillus subtilis infected with phage SP-15 were examined to investigate the metabolic origin of two of the unique components of the phage DNA: the component responsible for the unusually high buoyant density in CsCl and the unusual pyrimidine, 5-(4′, 5′-dihydroxypentyl) uracil (DHPU). Newly synthesized pulse-labeled DNA was light in buoyant density and shifted to the high density of mature phage DNA upon further incubation. Parental DNA was converted to a light-density intermediate form prior to replication. When labeled uracil, thymidine, or DHPU were added to infected cells, it was found that only uracil served as the precursor to DHPU and thymine in phage DNA. Analysis of the bases from hydrolyzed DNA of labeled phage or infected cells indicated that the uracil was incorporated into the DNA as such (presumably via deoxyuridine triphosphate) and later converted to DHPU and thymine at the macromolecular level. The sequence of events after phage infection appeared to be: (i) injection of parental DNA; (ii) conversion of parental DNA to a light form; (iii) DNA replication, yielding light DNA containing uracil; (iv) conversion of uracil to DHPU and thymine; and (v) addition of the heavy component.
机译：检测噬菌体SP-15感染的芽孢杆菌的培养物，研究了噬菌体DNA的两种独特组分的代谢来源：负责CSCL中异常高的浮力密度的组分和不寻常的嘧啶，5-（4'，5'-二羟基甲型基）Uracil（DHPU）。新合成的脉冲标记的DNA是浮力密度的光，并在进一步孵育时移至成熟噬菌体DNA的高密度。在复制之前将亲本DNA转化为光密度中间形式。当向感染细胞中添加尿嘧啶，胸苷或DHPU时，发现尿嘧啶只有DHPU的前体和噬菌体DNA中的胸腺嘧啶。来自标记噬菌体或受感染细胞的水解DNA的碱的分析表明，尿嘧啶掺入DNA中（可能通过脱氧尿苷三磷酸脱氧尿苷），后来在大分子水平上转化为DHPU和胸腺嘧啶。噬菌体感染后的事件序列似乎是：（i）注射父母DNA; （ii）将亲本DNA转化为光形式; （iii）DNA复制，产生含尿嘧啶的光DNA; （iv）将尿嘧啶转化为DHPU和胸腺嘧啶; （v）加入重组分。

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