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首页> 外文期刊>Journal of Virology >Infectivity and RNA Patterns as Functions of High- and Low-Dilution Passage of Murine Sarcoma-Leukemia Virus: Evidence for Autointerference Within an Oncornavirus Population
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Infectivity and RNA Patterns as Functions of High- and Low-Dilution Passage of Murine Sarcoma-Leukemia Virus: Evidence for Autointerference Within an Oncornavirus Population

机译：感染性和RNA模式作为鼠Sarcoma-Leukemia病毒的高和低稀释通道的功能：在oncornavirus人群中自动引用的证据

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Heterogeneity of buoyant density and RNA content of virions of Moloney murine leukemia-sarcoma complex [MSV (MLV)] was the result of passage at low dilution. Heterogeneous stocks revealed two major RNA components in the population, with the smaller component, apparent mol wt 4 × 106 to 5 × 106, becoming predominant upon serial passage at low dilution. Concomitantly, infectivity titers of both MLV and MSV decreased upon serial passage at low dilution. MSV (MLV) passaged at high dilution retained high titers and a rather homogeneous high-molecular-weight RNA population characteristic of high-buoyant-density virions. Interference of both MLV and MSV replication was demonstrated by employing mixed inocula containing both low- and high-dilution passage stocks of MSV (MLV). In contrast to results with MSV (MLV), MLV freed of MSV by limit dilution did not show heterogeneity of buoyant density or of RNA when propagated at low dilution.
机译：莫尼鼠白血病 - 肉瘤络合物[MSV（MLV）]的病毒血管的浮力密度和RNA含量的异质性是通过低稀释剂通过的结果。异质股揭示了群体中的两个主要RNA组分，具有较小的组分，表观摩尔WT 4×10 6 - 10 6 ，在低温下在连续通道时成为主要的稀释。同时，在低稀释的连续通道时，MLV和MSV的感染性滴度降低。在高稀释度上传递的MSV（MLV）保留高滴度和高浮力密度病毒群的相当均匀的高分子量RNA群。通过使用含有MSV（MLV）的低稀释通道股的混合接种物来证明MLV和MSV复制的干扰。与MSV（MLV）的结果相比，通过极限稀释的MLV释放MLV未显示在低稀释时在繁殖时浮力密度或RNA的异质性。

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