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首页> 外文期刊>Journal of Virology >Analysis of High-Molecular-Weight Ribonucleic Acid Associated with Intracisternal A Particles
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Analysis of High-Molecular-Weight Ribonucleic Acid Associated with Intracisternal A Particles

机译：与粒子颗粒相关的高分子量核糖核酸分析

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摘要

Intracisternal A particles, known primarily for their association with various tumors, have been shown to contain high-molecular-weight (HMW) ribonucleic acid (RNA) by velocity centrifugation, using linear glycerol gradients. This HMW RNA is sensitive to ribonuclease digestion and alkali treatment but is resistant to Pronase treatment. By a double-labeling experiment, HMW RNA was shown to be intrinsic to intracisternal A particles and not to have resulted from cytoplasmic polysomal RNA aggregation. By a reconstitution experiment, it was determined that the results were not due to C-type virus contamination. The synthesis of HMW RNA in intracisternal A particles is inhibited by actinomycin D and ethidium bromide. These observations emphasize that there are probably some taxonomic relationships between intracisternal A particles and oncogenic RNA viruses.
机译：颅内节颗粒主要用于与各种肿瘤相关的颗粒，已被证明通过使用线性甘油梯度含有速度离心的高分子量（HMW）核糖核酸（RNA）。这种HMW RNA对核糖核酸酶消化和碱处理敏感，但耐浸酶治疗。通过双标签实验，显示HMW RNA对颗粒具有内在的颗粒，而不是由细胞质多视性RNA聚集产生的。通过重构实验，确定结果不是由于C型病毒污染。颗粒中颗粒中HMW RNA的合成受到放线霉素D和溴化乙酰胺抑制。这些观察结果强调，脑内颗粒和致癌RNA病毒可能存在一些分类学关系。

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