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首页> 外文期刊>Microbiology >A Comparative Study on the Biochemical Bases of the Maximum Temperatures for Growth of Three Psychrophilic Micro-Organisms
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A Comparative Study on the Biochemical Bases of the Maximum Temperatures for Growth of Three Psychrophilic Micro-Organisms

机译：三种心理微生物生长的最大温度的生化基础的比较研究

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SurmmaryThree psychrophilic micro-organisms (strains of Arthrobacter, Candida and Corynebacterium erythrogenes) were capable of growth for a period when exponential-phase cultures in chemically defined media were transferred from temperatures at or near the optima for growth (20, 10 and 15°, respectively), to 37, 25 and 30°, respectively. The latter temperatures were 3–5° above the maxima for the growth of the organisms in freshly inoculated cultures. Growth at the higher temperatures was greatest with the Candida and least with the Arthrobacter. Cultures of the Arthrobacter and Candida grew when transferred back to the optimum temperatures for growth, after a lag which increased with the length of time that the cultures had spent at the higher temperatures. C. erythrogenes cultures grew almost immediately after they were transferred back to the optimum growth temperature. Growth of the organisms at the higher temperatures was not affected by supplementing cultures with bacteriological peptone and/or yeast extract. There was a rapid decline in the viability and in the rates of respiration of endogenous reserves and of exogenous glucose and pyruvate when Arthrobacter and Candida cultures were transferred to the higher temperatures. But with C. erythrogenes the respiratory activities were not so markedly affected by the change in incubation temperature, while the viability of this bacterium increased slightly after the transfer of cultures to the higher temperature. The activities of many of the tricarboxylic acid cycle enzymes in Arthrobacter and Candida were diminished after the transfer of organisms from the optimum temperature to one above the maximum for growth; but the activities of these enzymes in C. erythrogenes were less affected by the change in incubation temperature. There was no marked intracellular accumulation or excretion of ultraviolet-absorbing compounds by the organisms after the transfer of cultures to the higher temperatures. The results are discussed in relation to the biochemical factors which may determine the maximum temperatures for growth of these organisms.
机译：SurmmaryThree心理学微生物（关节杆菌的菌株，念珠菌和棒状杆菌的红细胞）能够在化学定义培养基中的指数相培养物中从最佳生长（20,10和15°，分别为37,25和30°。后者温度为最大值3-5°，用于新接种培养物中生物体的生长。较高温度的生长与念珠菌和至少与关节杆菌最少的较大。在滞后后转移到增长的最佳温度，随着培养物在较高温度下花费的时间长度而增加，念珠菌和念珠菌的培养物增长。 C.在转移到最佳生长温度后，红细胞培养物几乎立即生长。在较高温度下生长的生长不受含有细菌蛋白胨和/或酵母提取物的培养物的影响。活力迅速下降，以及内源储备的呼吸率和随后的葡萄糖和丙酮酸的呼吸，当花杆菌和念珠菌培养物转移到较高温度时。但是，通过C. eryThorenes，呼吸系统的呼吸系统活性不会受到培养温度变化的显着影响，而这种细菌的活力在将培养物转移到较高温度后略微增加。在从最佳温度从最佳温度转移到最大生长后，有机杆菌和念珠菌中许多三羧酸循环酶的活性减少到最大值以上;但是，通过培养温度变化，这些酶的活性较小。在将培养物转移到较高温度之后，生物体没有明显的细胞内积聚或紫外线吸收化合物排泄。结果讨论了可以确定这些生物体生长的最高温度的生化因子。

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