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首页> 外文期刊>The biochemical journal >N-Acetyl-β-glucosaminidases in human spleen
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N-Acetyl-β-glucosaminidases in human spleen

机译：N-乙酰基-β-葡糖胺酶在人脾中

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p1. The iN/i-acetyl-β-glucosaminidase of human spleen has been separated by gel electrophoresis into two components, an acidic form A and a basic form B. 2. The two forms are readily separated on DEAE-cellulose and have been concentrated 50-fold and sevenfold respectively. 3. They show similar iKsubm/sub/i values towards 4-methylumbelliferyl iN/i-acetyl-β-d-glucosaminide, and have the same pH optima when compared in citrate, phosphate or acetate buffers. They are inhibited to a similar extent by acetate, heparin, iN/i-acetylgalactosaminolactone, iN/i-acetyl-β-d-galactosamine and iN/i-acetyl-β-d-glucosamine. Specificity for C-4 orientation is not absolute and ip/i-nitrophenyl β-galactosaminide is also hydrolysed but at a rate only 11·6% of that for the corresponding glucosaminide. 4. iN/i-Acetyl-β-glucosaminidase B is stable over a wider pH range than is iN/i-acetyl-β-glucosaminidase A, and is less easily denatured by heat. 5. Tissue fractionation indicates that both the A and B forms are present in the lysosomal fraction, whereas the supernatant contains the A form only. 6. Evidence is presented to indicate that the A form contains a number of sialic acid residues./p
机译：> 1。人脾的 n - 乙酰-β-葡糖蛋白酶酶已通过凝胶电泳分为两个组分，酸性形式A和碱性形式B.2。在DEAE-纤维素上容易分离两种形式并分别集中50倍和七倍。 3.它们显示出类似的 k m 值朝向4-甲基umb蛋白酶1 n-β-d-葡糖胺素蛋白质，并且当时具有相同的pH值以柠檬酸盐，磷酸盐或乙酸盐缓冲液进行比较。它们被醋酸盐，肝素， - 乙酰丙基氨基甲烷酮， N-I> - 乙酰-β-D-半乳糖胺和 N-I> - 乙酰基抑制在相似程度上-β-D-葡糖胺。 C-4取向的特异性不是绝对的，并且 P - 硝基苯基β-半乳糖胺素也被水解但仅为相应氨糖苷的速率仅为11·6％。 4. N - 乙酰-β-葡糖胺酶B在较宽的pH范围内稳定，而不是 n-β-葡糖胺酶A，并且不容易通过热变性。组织分馏表明A和B形式存在于溶酶体级分中，而上清液仅含有形式。 6.提出了证据表明表单含有许多唾液酸残基。

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