...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of Virology >Early Abortive Lysis by Phage BF23 in Escherichia coli K-12 Carrying the Colicin Ib Factor
【24h】

Early Abortive Lysis by Phage BF23 in Escherichia coli K-12 Carrying the Colicin Ib Factor

机译：早期流产裂解噬菌体BF23在大肠杆菌K-12中携带肠道IB因子

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Growth of phage BF23 was restricted in Escherichia coli K-12 strains carrying a colicin I factor (ColIb); most infected cells lysed early without producing progeny phages. Either addition of chloramphenicol before phage infection or ultraviolet irradiation of phage prevented early abortive lysis, an indication that certain phage functions are required for this phenomenon. Very little or no phage-induced lysozyme was synthesized in the infected ColI+ cells. This result suggests that early abortive lysis was not due to the lysozyme action. A small fraction (0.05) of BF23-infected ColI+ cells showed normal phage growth. This “escaped growth” may reflect the physiological state of the host bacteria rather than the heterogeneity of the infecting phage. Host-controlled modification was not observed. A phage mutant, BF23hI, able to grow on ColI+ cells, was isolated and was characterized to be recessive to the wild-type BF23 in its ability to undergo early abortive lysis. Among the T series phages, T5 induced early abortive lysis, and growth of T5 was restricted upon infection to ColI+ cells. These results and the other observations, including the occurrence of phenotypic mixing between BF23 and T5, suggest that these two phages are related to each other even though the receptor sites for BF23 and T5 are apparently different.
机译：噬菌体BF23的生长受到携带耐粘酶I因子（COLIB）的大肠杆菌C大肠杆菌（Colib）;大多数受感染的细胞早期裂解而不产生后噬菌体。在噬菌体感染或噬菌体噬菌体前加入氯霉素，防止早期流血裂解，这表明这种现象需要某些噬菌体函数。在感染的大肠杆菌 + 细胞中合成了很少或没有噬菌体诱导的溶菌酶。该结果表明，早期流产裂解不是由于溶菌酶作用。 BF23感染的大肠杆菌 + 细胞的一小部分（0.05）显示正常噬菌体生长。这种“逃逸的生长”可以反映宿主细菌的生理状态，而不是感染噬菌体的异质性。未观察到宿主控制的修改。噬菌体突变体BF23HI，能够在大肠杆菌上生长，并被分离，其特征在于在野生型BF23中隐性，其能够进行早期流产裂解的能力。在T系列噬菌体噬菌体中，T5诱导的早期流动裂解，并且T5的生长受到感染对COLI + 细胞。这些结果和其他观察结果包括在BF23和T5之间发生表型混合的发生，表明这两个噬菌体彼此相关，即使BF23和T5的受体位点显然是不同的。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号