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首页> 外文期刊>Journal of Virology >ΦX-174 Bacteriophage Structural Mutants Which Affect Deoxyribonucleic Acid Synthesis
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ΦX-174 Bacteriophage Structural Mutants Which Affect Deoxyribonucleic Acid Synthesis

机译:φX-174影响脱氧核酸合成的噬菌体结构突变体

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Seven cistrons in ΦX-174 were identified and one in particular was studied intensively: cistron A, which is assigned a protein in the mature phage. Amber mutants in this cistron synthesize a new deoxyribonucleic acid (DNA) form in addition to circular phage DNA upon infection of the restrictive host. This DNA is linear, non-infectious, and single-stranded; it is formed from the phage strand of replicative form ΦX-174 DNA. These mutants produce two different defective particles in the restrictive host. One particle contains circular phage DNA but is not infectious; the other contains the new DNA form and is similar to the 70S particles found in wild-type phage lysates. The mutant A gene product acts independently of normal A protein upon mixed infection of the restrictive host with an A mutant and a mutant from any other cistron or wild type.
机译:φX-174中的七个传感器被鉴定,特别是一个集中研究:Cistron A,其在成熟噬菌体中分配蛋白质。在感染限制宿主后,该Cistron中的琥珀突变体除了圆形噬菌体DNA之外还合成新的脱氧核糖核酸(DNA)形式。该DNA是线性的,非感染性和单链;它由复制形式的φX-174 DNA的噬菌体链形成。这些突变体在限制宿主中产生两种不同的缺陷颗粒。一个颗粒含有圆形噬菌体DNA,但不传染;另一个含有新的DNA形式,类似于在野生型噬菌体裂解物中发现的70 S 颗粒。突变体在用突变体和突变体的混合感染的限制宿主混合感染突变体和突变体的情况下,基因产物独立于正常的蛋白质。

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