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首页> 外文期刊>日本作物學會紀事 >Studies on the Auxin Metabolism of Some Cereal Crops under Germination and Seedling Growth : III. Some experiments in vitro on the IAA-destroying system in the wheat seedling
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Studies on the Auxin Metabolism of Some Cereal Crops under Germination and Seedling Growth : III. Some experiments in vitro on the IAA-destroying system in the wheat seedling

机译：萌发和幼苗生长下一些谷物作物的蟾蜍蛋白代谢研究：III。小麦幼苗IAA破坏系统体外实验

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The mechanism of IAA-destroying system in the wheat seedling is discussed in this paper. The IAA-oxidase activity in the crude homogenate of dark-grown wheat seedlings is masked by the existence of the IAA-oxidase inhibitors in the homogenate, but the addition of H2O2 to the homogenate removes this masking and the high IAA-oxidase activity becomes visible. Then, the ferulic acid, one of the IAA-oxidase inhibitors, H2O2 and the dialysed enzyme solution from whet coleoptile were mixed and incubated for detecting the changes in UV-absorption spectrum of the mixture. As a result, it was observed that the ferulic acid in the mixture was oxidized by the H2O2-peroxidase system. The masking of IAA-oxidase activity in the crude homogenate is also removed by the addition of riboflavin (FR) or flavin mononucleotide (FMN) and light instead of H2O2, but the enhancement of IAA-destroying action by FR (FMN) and light is observed without enzyme preparation in the mixture. This sesult suggests that it is the non-enzymatic destruction of IAA. It was also observed that this non-enzymatic destruction of IAA by FR (FMN) and light is inhibited by IAA-oxidase inhibitors, phenolics, contained in wheat seedlings. From the above-mentioned results, the authors have supposed the scheme of IAA-destroying system in wheat seedling as shown in Fig. 5.
机译：本文讨论了小麦幼苗中IAA破坏系统的机制。通过在匀浆中存在IAA-氧化酶抑制剂的粗糙小麦幼苗的粗匀浆中的IAA-氧化酶活性，但是将H 2 O 2加入匀浆中除去该掩蔽，并且高IAA-氧化酶活性变得可见。然后，将阿魏酸，IAA-氧化酶抑制剂，H 2 O 2和透析酶溶液中的一种混合，并孵育，用于检测混合物的UV吸收光谱的变化。结果，观察到混合物中的阿魏酸通过H 2 O 2 - 过氧化物酶系统氧化。通过添加核黄素（FR）或黄蛋白单核苷酸（FMN）和光而不是H 2 O 2，也除去粗匀浆中IAA-氧化酶活性的掩蔽。（FMN）和光的增强在混合物中没有酶制剂观察。这位萨因表明它是IAA的非酶促破坏。还观察到，由Fr（FMN）和光的这种非酶促破坏由IAA-氧化酶抑制剂，酚醛菌抑制，含有小麦幼苗。根据上述结果，作者认为，如图5所示，在小麦幼苗中造成了IAA破坏系统的方案。5。

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