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首页> 外文期刊>Journal of Virology >Properties of Simian Virus 40 Rescued from Cell Lines Transformed by Ultraviolet-Irradiated Simian Virus 40
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Properties of Simian Virus 40 Rescued from Cell Lines Transformed by Ultraviolet-Irradiated Simian Virus 40

机译：Simian病毒40的性质从紫外线辐照的Simian病毒40转换的细胞系中救出

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Simian virus 40 (SV40) strains have been rescued from various clonal lines of mouse kidney cells that had been transformed by ultraviolet (UV)-irradiated SV40. To learn whether some of the rescued SV40 strains were mutants, monkey kidney (CV-1) cells were infected with the rescued virus strains at 37 C and at 41 C. The SV40 strains studied included strains rescued from transformed cell lines classified as “good,” “average,” “poor,” and “rare” yielders on the basis of total virus yield, frequency of induction, and incidence of successful rescue trials. Four small plaque mutants isolated from “poor” yielder lines and fuzzy and small plaque strains isolated from an “average” and a “good” yielder line, respectively, were among the SV40 strains tested. Virus strains rescued from all classes of transformed cells were capable of inducing the transplantation antigen, and they induced the intranuclear SV40-T-antigen, thymidine kinase, deoxyribonucleic acid (DNA) polymerase, and cellular DNA synthesis at 37 C and at 41 C. With the exception of four small plaque strains rescued from “poor” yielders, the rescued SV40 strains replicated their DNA and formed infectious virus with kinetics similar to parental SV40 at either 37 or 41 C. The four exceptional strains did replicate at 37 C, but replication was very poor at 41 C. Thus, only a few of the rescued virus strains exhibited defective SV40 functions in CV-1 cells. All of the virus strains rescued from the “rare” yielder lines were similar to parental SV40. Several hypotheses consistent with the properties of the rescued virus strains are discussed, which may account for the significant variations in virus yield and frequency of induction of the transformed cell lines.
机译：Simian病毒40（SV40）菌株已从紫外（UV）式SV40转化的小鼠肾细胞的各种克隆物系中拯救。要了解一些救助的SV40菌株是否是突变体，猴肾（CV-1）细胞在37℃和41℃下感染救助的病毒菌株。研究的SV40菌株包括从分类为“好的转化细胞系的菌株，“”平均“，”差“和”罕见“产量基于总病毒产量，诱导频率和成功救援试验的发病率。从“平均”和“良好”的yielder分别分离的“差”yielder线和模糊和小斑块菌株分别隔离的四个小斑块突变体分别在测试的SV40菌株中。从所有类转化细胞中拯救的病毒菌株能够诱导移植抗原，并且它们诱导核核SV40-T-抗原，胸苷激酶，脱氧核糖核酸（DNA）聚合酶和37℃和41℃的细胞DNA合成。除了从“贫困”产量救出的小斑块菌株外，救出的SV40菌株将其DNA复制，并将传染性病毒与37或41℃的亲本SV40类似的动力学复制。四种特殊菌株在37℃下复制，但是在41℃下复制非常差。因此，只有剩余的救助病毒菌株在CV-1细胞中表现出缺陷的SV40功能。从“罕见”的yielder系中拯救的所有病毒菌株都与父母SV40类似。讨论了与救助病毒株的性质一致的几个假设，其可能考虑到病毒产量和转化细胞系的诱导频率的显着变化。

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