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T-Even Bacteriophage-Tolerant Mutants of Escherichia coli B I. Isolation and Preliminary Characterization

机译:大肠杆菌BI的T型甚至噬菌体耐受性突变体。分离和初步表征

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A general procedure is described for isolation of T-even phage-tolerant mutants of Escherichia coli. Two such mutants of E. coli B have been examined in some detail. These mutants adsorb T-even phages but are unable to release viable progeny. Under certain conditions, viability of the cells is completely unaffected by phage infection in one mutant, and there is but a slight decrease in colony-forming ability in the other. Phage deoxyribonucleic acid (DNA) is injected into these cells, as shown by the formation of phage-specific enzymes, but it is not degraded to acid-soluble material. Some phage DNA replication occurs in both strains. The mutants are both more resistant to ultraviolet light than is the parent strain.
机译:描述了一般方法,用于分离大肠杆菌的T型均耐受性突变体。 e的两个这样的突变体。已经详细检查了大肠杆菌 b。这些突变体吸附T-偶数,但不能释放可行的后代。在某些条件下,细胞的活力完全不受一个突变体中感染的噬菌体感染的影响,并且在另一个突变体中具有略微降低。噬菌体将脱氧核糖核酸(DNA)注入这些细胞中,如噬菌体特异性酶的形成所示,但它不会降解到酸溶质的材料中。在两个菌株中发生一些噬菌体DNA复制。突变体均耐紫外光比是亲本菌株更耐紫外线。

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