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首页> 外文期刊>Journal of Virology >Genetic Characteristics of Conditional Lethal Mutants of Vesicular Stomatitis Virus Induced by 5-Fluorouracil, 5-Azacytidine, and Ethyl Methane Sulfonate
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Genetic Characteristics of Conditional Lethal Mutants of Vesicular Stomatitis Virus Induced by 5-Fluorouracil, 5-Azacytidine, and Ethyl Methane Sulfonate

机译：用5-氟尿嘧啶，5-氮杂胞苷和乙基甲磺酸盐诱导的囊泡口炎病毒有条件致命突变体的遗传特征

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One hundred and seventy-five temperature-sensitive (ts) mutants of vesicular stomatitis virus (type Indiana-C) induced by 5-fluorouracil (FU), 5-azacytidine (ACR), and ethyl methane sulfonate (EMS) have been assigned to four complementation groups by a qualitative test. Group I contains 151 mutants; group II, 2 mutants; group III, 1 mutant; and group IV, 15 mutants; 6 are unclassified. FU was much more effective as a mutagen than either ACR or EMS. The proportion of the mutants belonging to groups I and IV, however, was similar in the case of all three mutagens. Fifteen mutants from groups I and IV have been used to obtain quantitative complementation data. Both groups appear to be homogeneous. Complementation yields increase with increasing multiplicity, but the number of particles per cell required to elicit maximal complementation is small. The pattern of genetic recombination parallels that of complementation. No recombination could be detected in crosses within group I (<0.001%) or group IV (<0.07%), whereas recombination (0.31 to 3.4%) was observed in crosses between groups I and IV. Recombination frequency did not increase with multiplicity above an input of 0.6 plaque-forming units per cell. Many group I mutants have very low reversion rates, and BHK 21 clone 13 cells infected with one of these mutants have been “cured” of infection by prolonged exposure at the restrictive temperature.
机译：由5-氟尿嘧啶（FU），5-氮杂胞苷（ACR）和乙基甲磺酸乙酯致癌诱导的百分之百和七十五的温度敏感（印第安纳型-c）突变体突变体（EMS）已通过定性测试分配给四个互补组。群体含有151个突变体;第II组，2个突变体;第三组，1个突变体;和第四组，15个突变体; 6是未分类的。傅比ACR或EMS更有效。然而，在所有三种诱变中的情况下，属于群体I和IV的突变体的比例。来自I和IV组的十五个突变体已被用于获得定量互补数据。两组似乎都是均匀的。互补产率随着多重性的增加而增加，但引出最大互补所需的每个单元的粒子数量很小。族遗传重组与互补的模式。在I族（<0.001％）或第IV组（<0.07％）内没有重组可以在十字中检测到，而在I和IV基团之间的交叉中观察到重组（0.31-3.4％）。重组频率在每种细胞的0.6个斑块形成单元的输入上方没有增加。许多组I突变体具有非常低的回复率，并且通过在限制温度下长时间暴露，感染其中一种突变体感染的BHK 21克隆13细胞已被“固化”。

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    《Journal of Virology》 |1970年第5期|共9页
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    C. R. Pringle;

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  • 收录信息 美国《科学引文索引》(SCI);
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