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首页> 外文期刊>Journal of Virology >Identification of the Simian Virus 40 Which Replicates When Simian Virus 40-Transformed Human Cells Are Fused with Simian Virus 40-Transformed Mouse Cells or Superinfected with Simian Virus 40 Deoxyribonucleic Acid
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Identification of the Simian Virus 40 Which Replicates When Simian Virus 40-Transformed Human Cells Are Fused with Simian Virus 40-Transformed Mouse Cells or Superinfected with Simian Virus 40 Deoxyribonucleic Acid

机译：鉴定Simian病毒40，当Simian病毒40转化的人体细胞与Simian病毒40转化的小鼠细胞融合或用Simian病毒40脱氧核糖核酸进行超育

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Simian virus 40 (SV40) was rescued from heterokaryons of transformed mouse and transformed human cells. To determine whether the rescued SV40 was progeny of the SV40 genome resident in the transformed mouse cells, the transformed human cells, or both, rescue experiments were performed with mouse lines transformed by plaque morphology mutants of SV40. The transformed mouse lines that were used yielded fuzzy, small-clear, or large-clear plaques after fusion with CV-1 (African green monkey kidney) cells. The transformed human lines that were used did not release SV40 spontaneously or after fusion with CV-1 cells. From each mouse-human fusion mixture, only the SV40 resident in the transformed mouse cells was recovered. Fusion mixtures of CV-1 and transformed mouse cells yielded much more SV40 than those from transformed human and transformed mouse cells. The rate of SV40 formation was also greater from monkey-mouse than from human-mouse heterokaryons. Deoxyribonucleic acid (DNA) from SV40 strains which form fuzzy, largeclear, or small-clear plaques on CV-1 cells was also used to infect monkey (CV-1 and Vero), normal human, and transformed human cell lines. The rate of virion formation and the final SV40 yields were much higher from monkey than from normal or transformed human cells. Only virus with the plaque type of the infecting DNA was found in extracts from the infected cells. Two uncloned sublines of transformed human cells [W18 Va2(P363) and WI38 Va13A] released SV40 spontaneously. Virus yields were not appreciably enhanced by fusion with CV-1 cells. However, clonal lines of W18 Va2(P363) did not release SV40 spontaneously or after fusion with CV-1 cells. In contrast, several clonal lines of WI38 Va13A cells did continue to shed SV40 spontaneously.
机译：Simian病毒40（SV40）从转化的小鼠和转化的人细胞的异质子救出。为了确定救助的SV40是否是驻留在转化的小鼠细胞中的SV40基因组的后代，转化的人体细胞或两者，用SV40的斑块形态突变体转化的小鼠线进行救援实验。使用CV-1（非洲绿色猴子肾脏）细胞融合后，使用的转化鼠标线产生模糊，小清晰或大清晰的斑块。使用的转化的人系没有自发地释放SV40或融合后的CV-1细胞。从每种小鼠 - 人融合混合物中，回收仅驻留在转化的小鼠细胞中的SV40。 CV-1和转化的小鼠细胞的融合混合物产生的SV40比来自转化的人和转化的小鼠细胞的小鼠更高。猴鼠的SV40形成率也比来自人小鼠异质官能的更大。来自SV40菌株的脱氧核糖核酸（DNA），其在CV-1细胞上形成模糊，大型斑块或小透明斑块，也用于感染猴子（CV-1和Vero），正常的人和转化的人细胞系。猴子形成和最终SV40产量的速率与来自正常或转化的人细胞的猴子高得多。在感染细胞的提取物中发现了具有斑块类型的感染DNA的病毒。转化的人细胞的两个未遗漏的载体[W18 VA2（P363）和Wi38 Va13a]自发释放SV40。通过用CV-1细胞融合，病毒产量没有明显增强。然而，W18Va2（p363）的克隆线不会自发地释放SV40或与CV-1细胞融合之后。相反，Wi38 Va13a细胞的几条克隆线仍然继续自发地脱落SV40。

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