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首页> 外文期刊>Journal of Virology >LPP-1 Infection of the Blue-Green Alga Plectonema boryanum II. Viral Deoxyribonucleic Acid Synthesis and Host Deoxyribonucleic Acid Breakdown
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LPP-1 Infection of the Blue-Green Alga Plectonema boryanum II. Viral Deoxyribonucleic Acid Synthesis and Host Deoxyribonucleic Acid Breakdown

机译:LPP-1蓝绿藻Plectonema博伊姆的感染。病毒脱氧核糖核酸合成和宿主脱氧核糖核酸分解

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Host and viral deoxyribonucleic acid (DNA) metabolism in LPP-1-infected Plectonema boryanum was studied by equilibrium centrifugation in CsCl gradients. Approximately 50% of the host DNA is degraded to acid-soluble material between 3 and 7 hr after infection. Most of the acid-soluble product is reincorporated into viral DNA. Incorporation of exogenous 3H-adenine into viral DNA can be detected very early after infection (within the first 2 hr), but the bulk of viral DNA synthesis occurs between 6 and 8 hr. Both the breakdown of host DNA and the synthesis of viral DNA require protein synthesis during the first few hours of infection.
机译:通过CSCL梯度的平衡离心研究了LPP-1感染的 Plectonema Boryanuma-em>中的宿主和病毒脱氧核糖核酸(DNA)代谢。感染后,约50%的宿主DNA降解到3至7小时之间的酸溶质材料。大多数酸溶质产物被重新掺入病毒DNA。在感染后,可以在感染后(在前2小时内)掺入外源性 3 H-腺嘌呤,但在6至8小时之间发生大部分病毒DNA合成。宿主DNA的分解和病毒DNA的合成需要在感染的前几个小时内蛋白质合成。

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