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首页> 外文期刊>Journal of Virology >Inactivation of Nocardiophages ΦC and ΦEC by Extracts of Bacteriophage-Attachable Cells
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Inactivation of Nocardiophages ΦC and ΦEC by Extracts of Bacteriophage-Attachable Cells

机译：通过噬菌体可连接细胞提取物的NocardiophagesφC和φec的失活

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Cultures of several species of Nocardia, including N. erythropolis Mat-Ce and Mat-cE mating strains, were extracted with solvents in an attempt to isolate an inactivating complex for nocardiophages ΦC and ΦEC. Ethanol was the only solvent found effective in solubilizing an inhibitory substance. Inactivating extracts were obtained from the cells of all species to which the phage were able to attach. After extraction of whole cells or cell wall preparations, the phage could not effectively attach to them. Both phages ΦC and ΦEC were inactivated by the same complex. However, phage ΦEC inactivation was 10-fold greater than ΦC inactivation. The velocity of inactivation was about 4.1 × 102 plaque-forming units per microgram per minute for ΦC and 1.1 × 103 plaque-forming units per microgram per minute for phage ΦEC. The cell extracts required divalent cations for phage inactivation. The inhibitory capacity of the cell extracts was reduced or lost by the activity of proteolytic enzymes, Tween 80, 2-mercaptoethanol, thymol, and sodium lauryl sulfate. Boiling the extract for 10 min did not alter its activity. The inactivating substance was postulated to be a lipoprotein of considerable complexity, unique in the ease with which it is solubilized from host cells by ethanol.
机译：若干物种 Nocardia ，包括 n。用溶剂萃取红细胞Ce和Mat-Ce配合菌株，试图分离用于NocardiophapyφC和φec的灭活复合物。乙醇是唯一有效溶解抑制物质的溶剂。从噬菌体能够附着的所有物种的细胞中获得灭活提取物。在提取全细胞或细胞壁制剂后，噬菌体无法有效地连接到它们。两个噬菌体φc和φec都是通过相同的复合物灭活。然而，噬菌体φec灭活是大于φC灭活的10倍。 φC每分钟的每分钟每分钟的φC每分钟的每分钟的速度为约4.1×10 2单位。噬菌体φec每分钟每分钟每分钟的1.1×10 3 斑块形成单位。细胞提取物用于噬菌体灭活所需的二价阳离子。通过蛋白水解酶，吐温80,2-巯基乙醇，胸腺酚和月桂基硫酸钠的活性降低或损失细胞提取物的抑制能力。沸腾提取物10分钟并没有改变其活动。将灭活物质假设为具有相当大的复杂性的脂蛋白，其易于通过乙醇从宿主细胞中溶解的容易性。

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