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首页> 外文期刊>Journal of Virology >Proteins of Vesicular Stomatitis Virus and of Phenotypically Mixed Vesicular Stomatitis Virus-Simian Virus 5 Virions
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Proteins of Vesicular Stomatitis Virus and of Phenotypically Mixed Vesicular Stomatitis Virus-Simian Virus 5 Virions

机译：囊泡口炎病毒的蛋白质和表型混合的囊泡口炎病毒-imian病毒5病毒

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The identity of the glycoprotein of vesicular stomatitis virus (VSV) as the spike protein has been confirmed by the removal of the spikes with a protease from Streptomyces griseus, leaving bullet-shaped particles bounded by a smooth membrane. This treatment removes the glycoprotein but does not affect the other virion proteins, apparently because they are protected from the enzyme by the lipids in the viral membrane. The proteins of phenotypically mixed, bullet-shaped virions produced by cells mixedly infected with VSV and the parainfluenza virus simian virus 5 (SV5) have been analyzed by polyacrylamide gel electrophoresis. These virions contain all the VSV proteins plus the two SV5 spike proteins, both of which are glycoproteins. The finding of the SV5 spike glycoproteins on virions with the typical morphology of VSV indicates that there is not a stringent requirement that only the VSV glycoprotein can be used to form the bullet-shaped virion. On the other hand, the SV5 nucleocapsid protein and the major non-spike protein of the SV5 envelope were not detected in the phenotypically mixed virions, and this suggests that a specific interaction between the VSV nucleocapsid and regions of the cell membrane which contain the nonglycosylated VSV envelope protein is necessary for assembly of the bullet-shaped virion.
机译：通过从链霉菌族GISTESS 从蛋白酶去除穗状体的穗状体蛋白作为尖峰蛋白的糖蛋白的同一性已经证实了穗状体蛋白质，从链霉菌霉菌中，留下由光滑膜界定的子弹形颗粒。该处理除去糖蛋白，但不影响其他病毒蛋白，显然是因为它们通过病毒膜中的脂质从酶保护。通过聚丙烯酰胺凝胶电泳分析了通过混合的细胞产生的细胞产生的细胞产生的细胞和Parainfluenza病毒Simian病毒5（SV5）产生的表型混合的子弹状病毒蛋白。这些病毒粒子含有所有VSV蛋白质加上两个SV5穗蛋白，两者都是糖蛋白。具有VSV的典型形态的病毒粒子上的SV5穗糖蛋白的发现表明，只有VSV糖蛋白可以使用VSV糖蛋白来形成子弹形病毒虫。另一方面，在表型混合的病毒粒子中未检测到SV5核衣壳蛋白和SV5封装的主要非尖峰蛋白，这表明VSV核衣壳和细胞膜的区域之间的特异性相互作用，其含有氮胶质化的细胞膜VSV包络蛋白是组装子弹形病毒虫所需的。

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