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首页> 外文期刊>Journal of Virology >Number and Molecular Weights of Foot-and-Mouth Disease Virus Capsid Proteins and the Effects of Maleylation
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Number and Molecular Weights of Foot-and-Mouth Disease Virus Capsid Proteins and the Effects of Maleylation

机译：口蹄疫病毒衣壳胶囊蛋白的数量和分子量及裂解的影响

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Evidence was obtained by gel electrophoresis that foot-and-mouth disease virus (FMDV) type A12 protein migrates mainly in a zone corresponding to polypeptide(s) approximately 25,000 daltons in molecular weight. Additional minor components were observed, four with molecular weights ranging from 10,000 to 22,500 daltons and one with a molecular weight of 37,500 daltons. The minor components comprised about 10% of the total protein and were present in variable amounts. The 75S empty capsids contained primarily 25,000-, 37,500- and 50,000-dalton zones. These molecular weights were estimated by polyacrylamide gel electrophoresis in sodium dodecyl sulfate versus proteins of known molecular weight, including poliovirus and vesicular stomatitis virus proteins. Maleylation of the amino residues of FMDV protein solubilized it to about 5 to 10 mg/ml in aqueous, nondenaturing solvents. This permitted molecular weights to be estimated also by gel filtration. Maleylation of 70% of the available amino groups of the FMDV protein produced heat and sodium dodecyl sulfate-stable polymeric aggregates of 10 to 20% of the 25,000-dalton zone. It also resulted in an increase in the molecular weight of this zone by an amount equivalent (ca. 1,000) to that expected from the added maleyl residues.
机译：通过凝胶电泳获得的证据，即脚口疾病病毒（FMDV）A型A 12 蛋白主要迁移在与多肽对应于多肽的区域中，其分子量约为25,000道尔顿。观察到另外的次要组分，其中四种分子量范围为10,000至22,500道尔顿，其中分子量为37,500道尔顿。次要组分包含约10％的总蛋白质，并以可变量存在。 75 S 空囊囊主要包含25,000-，37,500和50,000-dalton区。通过已知分子量的十二烷基硫酸钠与蛋白质，包括脊髓灰质炎病毒和囊泡口腔炎病毒蛋白，这些分子量估计这些分子量。 FMDV蛋白的氨基残基的雄性化溶解在水性，不明确的溶剂中约5-10mg / ml。通过凝胶过滤估计这种允许的分子量。 FMDV蛋白质的70％可用氨基的雄性化产生的热量和十二烷基硫酸钠稳定的聚合物聚集体为25,000-Dalton区的10％至20％。它还导致该区的分子量增加了当量（CA.1,000）至预期来自添加的马来基残基的量。

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