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首页> 外文期刊>Journal of Virology >Bacteriophage T4 Inhibits Colicin E2-Induced Degradation of Escherichia coli Deoxyribonucleic Acid I. Protein Synthesis-Dependent Inhibition
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Bacteriophage T4 Inhibits Colicin E2-Induced Degradation of Escherichia coli Deoxyribonucleic Acid I. Protein Synthesis-Dependent Inhibition

机译：噬菌体T4抑制科尼蛋白E2诱导的大肠杆菌脱氧核糖核酸I.蛋白质合成依赖性抑制

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摘要

The deoxyribonucleic acid (DNA) of Escherichia coli B is converted by colicin E2 to products soluble in cold trichloroacetic acid; we show that this DNA degradation (hereafter termed solubilization) is subject to inhibition by infection with bacteriophage T4. At least two modes of inhibition may be differentiated on the basis of their sensitivity to chloramphenicol. The following observations on the inhibition of E2 by phage T4 in the absence of chloramphenicol are described: (i) Simultaneous addition to E. coli B of E2 and a phage mutated in genes 42, 46, and 47 results in a virtually complete block of the DNA solubilization normally induced by E2; the mutation in gene 42 prevents phage DNA synthesis, and the mutations in genes 46 and 47 block a late stage of phage-induced solubilization of host DNA. (ii) This triple mutant inhibits equally well when added at any time during the E2-induced solubilization. (iii) Simultaneous addition to E. coli B of E2 and a phage mutated only in gene 42 results in extensive DNA solubilization, but the amount of residual acid-insoluble DNA (20 to 25%) is more characteristic of phage infection than of E2 addition (5% or less). (iv) denA mutants of phage T4 are blocked in an early stage (endonuclease II) of degradation of host DNA; when E2 and a phage mutated in both genes 42 and denA are added to E. coli B, extensive solubilization of DNA occurs with a pattern identical to that observed upon simultaneous addition of E2 and the gene 42 mutant. (v) However, delaying E2 addition for 10 min after infection by this double mutant allows the phage to develop considerable inhibition of E2. (vi) Adsorption of E2 to E. coli B is not impaired by infection with phage mutated in genes 42, 46, and 47. In the presence of chloramphenicol, the inhibition of E2 by the triple-mutant (genes 42, 46, and 47) still occurs, but to a lesser extent.
机译：大肠杆菌Coli B的脱氧核糖核酸（DNA）由肠E2转化为溶于冷三氯乙酸的产物;我们表明该DNA降解（以下称为溶解）通过用噬菌体T4感染受到抑制。至少两种抑制模式可以基于它们对氯霉素的敏感性来分化。描述了在没有氯霉素的情况下通过噬菌体T4抑制E2的以下观察结果：（i）与 E同时加入。 E2的COLI B和在基因42,46和47中突变的噬菌体导致通常由E2诱导的DNA溶解的几乎完全嵌段;基因42中的突变可防止噬菌体DNA合成，并且基因46和47中的突变阻断噬菌体诱导的宿主DNA的溶解的后期。 （ii）当在E2诱导的溶解期间在任何时间添加时，该三重突变体同样抑制。 （iii）同时添加到 e。 E2的Coli B仅在基因42中突变的噬菌体导致广泛的DNA溶解，但残留酸 - 不溶性DNA（20至25％）的量比E2加入更具特征（20至25％）比E2加入更具特征（5％或更少）。 （iv）噬菌体T4的突变体在宿主DNA的降解的早期阶段（内切核酸酶II）中封闭;当E2和在基因42和 Dena 中突变的噬菌体加入到 E中时。 Coli B，通过与同时加入E2和基因42突变体观察到的图案发生广泛的DNA溶解。 （v）然而，通过该双突变体感染后10分钟的延迟E2加入允许噬菌体产生相当大的E2抑制。 （vi）将E2吸附到 e。 Coli B不会通过在42,46和47中的噬菌体突变的噬菌体感染而受损。在氯霉素存在下，仍然发生三重突变体（基因42,46和47）e2的抑制，但在较小程度上。

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