• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>The biochemical journal >Evaluation of the rate-limiting steps in the pathway of glucose metabolism in kidney cortex of normal, diabetic, cortisone-treated and growth hormone-treated rats
【24h】

Evaluation of the rate-limiting steps in the pathway of glucose metabolism in kidney cortex of normal, diabetic, cortisone-treated and growth hormone-treated rats

机译：肾脏皮质血糖新陈代谢途径评价阶段评价正常，糖尿病，治疗和生长激素治疗大鼠的肾脏皮质途径

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

p1. The activities of gluconeogenic and glycolytic enzymes and the concentrations of citrate, ammonia, amino acids, glycogen, glucose 6-phosphate, acetyl-CoA, lactate and pyruvate were measured in kidney cortex of normal, diabetic, cortisone-treated and growth hormone-treated rats. 2. In kidney cortex of diabetic, cortisone-treated and growth hormone-treated rats the activities of glucose 6-phosphatase (EC 3.1.3.9), fructose 1,6-diphosphatase (EC 3.1.3.11) and phosphopyruvate carboxylase (EC 4.1.1.32) were increased. 3. The activities of glutamate dehydrogenase (EC 1.4.1.3), alanine aminotransferase (EC 2.6.1.2), aspartate aminotransferase (EC 2.6.1.10) and pyruvate carboxylase (EC 6.4.1.1) were increased in diabetic and cortisone-treated rats. In growth hormone-treated rats the activity of aspartate aminotransferase was depressed but those of the other three enzymes were unchanged. 4. The activity of hexokinase (EC 2.7.1.1) was not altered in any of these conditions. Phosphofructokinase (EC 2.7.1.11) activity was depressed only in growth hormone-treated rats. Pyruvate kinase (EC 2.7.1.40) activity was depressed in cortisone-treated and growth hormone-treated rats but unchanged in diabetic rats. 5. Amino acids, acetyl-CoA and glucose 6-phosphate contents were increased in rat kidneys in all these three conditions. Ammonia content was increased in diabetic and cortisone-treated rats but was markedly diminished in growth hormone-treated rats. 6. The [lactate]/[pyruvate] ratio was elevated in diabetic and cortisone-treated rats but unchanged in growth hormone-treated rats. Citrate content was increased in the kidney cortex of diabetic and growth hormone-treated rats but was unchanged in cortisone-treated rats. The activity of ATP citrate lyase (EC 4.1.3.8) was depressed in diabetic and growth hormone-treated rats but was increased in cortisone-treated rats. 7. Glycogen content was moderately elevated in growth hormone-treated rats and markedly elevated in diabetic rats, whereas no change in glycogen content was observed in cortisone-treated rats. Glycogen synthetase (EC 2.4.1.11) activity was unchanged in all these three conditions. Phosphorylase (EC 2.4.1.1) activity was not affected in cortisone-treated rats but was depressed in diabetic and growth hormone-treated rats./p
机译：> 1。肾皮层正常，糖尿病，可科西酮处理和生长激素处理的肾皮质，测定了葡糖苷和糖醛酶的活性，氨基酸，氨，氨基酸，糖原，葡萄糖6-磷酸酯，乙酰酸盐，乳酸和丙酮酸盐老鼠。 2.在糖尿病糖尿病肾皮层中，可可龙治疗和生长激素处理的大鼠葡萄糖6-磷酸酶（EC 3.1.3.9）的活性，果糖1,6-二磷酸酶（EC 3.1.3.11）和磷络物羧化酶（EC 4.1。 1.32）增加。 3.在糖尿病和皮质酮处理的大鼠中增加了谷氨酸脱氢酶（EC 1.4.1.3），丙氨酸氨基转移酶（EC 2.6.1.2），天冬氨酸氨基转移酶（EC 2.6.1.10）和丙酮酸羧基（EC 6.4.1.1）。在生长激素处理的大鼠中，抑制了天冬氨酸氨基转移酶的活性，但其他三种酶的活性不变。 4.六酮酶（EC 2.7.1.1）的活性在任何这些条件下没有改变。仅在生长激素处理的大鼠中抑制磷蛋白酶（EC 2.7.1.11）活性。丙酮酸激酶（EC 2.7.1.40）在可可酮处理和生长激素处理的大鼠中抑制了活性，但在糖尿病大鼠中保持不变。在所有这三种条件下，大鼠肾癌中氨基酸，乙酰辅酶和葡萄糖6-磷酸含量增加。糖尿病和皮质治疗大鼠氨含量增加，但在生长激素处理的大鼠中显着降低。 6. [乳酸] / [丙酮酸]比例在糖尿病和可可酮处理的大鼠中升高，但在生长激素处理的大鼠中保持不变。在糖尿病和生长激素治疗的大鼠的肾皮层中含有柠檬酸盐含量增加，但在可可酮处理的大鼠中不变。 ATP柠檬酸盐酶（EC 4.1.3.8）的活性在糖尿病和生长激素处理的大鼠中抑制，但在可可酮处理的大鼠中增加。 7.糖原含量在生长激素处理的大鼠中适度升高，糖尿病大鼠显着升高，而在可可酮处理的大鼠中观察到糖原含量的变化。在所有这三种条件下，糖原合成酶（EC 2.4.1.11）活性不变。磷酸化酶（EC 2.4.1.1）活性在可可酮处理的大鼠中不受影响，但在糖尿病和生长激素处理的大鼠中抑制。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号