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首页> 外文期刊>The biochemical journal >Glycolytic and gluconeogenic enzyme activities in parenchymal and non-parenchymal cells from mouse liver
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Glycolytic and gluconeogenic enzyme activities in parenchymal and non-parenchymal cells from mouse liver

机译：来自小鼠肝脏的实质和非实质细胞的糖酵解和葡糖来酶活性

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p1. Parenchymal cells have been prepared from mouse liver by enzymic and mechanical means. 2. The dry weights, protein and DNA contents of these cells have been determined. 3. Mouse liver ‘M-’ and ‘L-type’ pyruvate kinases have been prepared free of contamination with each other; their kinetic properties have been examined and a method has been developed for their assay in total liver homogenates. 4. Recoveries of phosphoglycerate kinase, lactate dehydrogenase and phosphofructokinase in enzymically prepared cells indicate that little, if any, cytoplasmic protein is lost during preparation. 5. Parenchymal cells exhibit a very substantial increase in the activity ratio of glucokinase to hexokinase over that in total liver homogenate; in three out of eight experiments, hexokinase activity was undetectable. 6. ‘L-type’ pyruvate kinase alone occurs in the parenchymal cell. Non-parenchymal cells are characterized by the presence of ‘M-type’ activity only. 7. Parenchymal cells contain both glucose 6-phosphatase and fructose 1,6-diphosphatase. The non-parenchymal fraction appears to contain fructose 1,6-diphosphatase, but is devoid of glucose 6-phosphatase. 8. No aldolase A was detectable in the whole liver. Aldolase B occurs in both parenchymal and non-parenchymal tissue. 9. Parenchymal cells prepared by mechanical disruption of mouse liver with 20% polyvinyl alcohol exhibit a similar enzyme profile to those prepared enzymically. 10. The methodology involved in the preparation of isolated liver cells is discussed. The importance of the measurement of several parameters as criteria for establishing the viability of parenchymal cells is stressed. 11. The metabolic implications of the results in the present study are discussed./p
机译：> 1。实质细胞已由酶和机械手段由小鼠肝脏制备。已经确定了这些细胞的干重，蛋白质和DNA含量。 3.小鼠肝脏'M-'和'L型'丙酮酸激酶已由彼此不含污染;已经检查了它们的动力学性质，并且已经在总肝脏匀浆中开发了它们的测定。 4.磷酸糖激酶的回收率，酶制备的细胞中的乳酸脱氢酶和磷化氨基酶表明，如果有的话，如果有任何细胞质蛋白在制备期间损失。 2.实质细胞表现出葡萄糖酮酶的活性比在总肝脏匀浆中的活性比的非常大幅增加;在八个实验中的三种中，六酮酶活性是不可检测的。 6.“L型”丙酮酸激酶单独发生在实质细胞中。非实质细胞的特征在于仅存在'm型'活性。 7.实质细胞含有葡萄糖6-磷酸酶和果糖1,6-二磷酶。非实质馏分似乎含有果糖1,6-二磷酸酯，但是没有葡萄糖6-磷酸酶。 8.在整个肝脏中可以检测到醛糖酶A.醛糖酶B发生在实质和非实质组织中。 9.通过使用20％聚乙烯的小鼠肝脏机械破坏制备的实质细胞，其具有与酶制备的那些类似的酶分布。讨论了涉及分离肝细胞的制备的方法。施加压力测量几个参数作为建立实质细胞的可行性的标准的重要性。 11.讨论了本研究结果的代谢影响。

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