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首页> 外文期刊>The biochemical journal >Soluble adenosine diphosphate glucose–α-1,4-glucan α-4-glucosyltransferases from spinach leaves
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Soluble adenosine diphosphate glucose–α-1,4-glucan α-4-glucosyltransferases from spinach leaves

机译：来自菠菜叶的可溶性腺苷二磷酸二磷酸二磷酸葡萄糖-α-1,4-葡聚糖α-4-葡糖基转移酶

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pMultiple forms of ADP-glucose–α-1,4-glucan α-4-glucosyltransferase were obtained from spinach leaves by gradient elution from a DEAE-cellulose column. In the presence of high concentrations of some salts and bovine serum albumin, unprimed activity was found in one (transglucosylase III) of the four fractions eluted from the column. In addition to having unprimed activity, transglucosylase III had a lower iKsubm/sub/i for ADP-glucose, a much higher iKsubm/sub/i for oyster glycogen, greater heat sensitivity and lower affinity for maltose, maltotriose and amylopectin β-limit dextrin than fractions I, II and IV. In addition, the kinetics at low concentrations of amylose, amylopectin and rabbit liver glycogen were non-linear for transglucosylase III. The properties of transglucosylases I, II and IV were generally similar to each other. Rates of the unprimed reaction at physiological concentrations of ADP-glucose were greater than those found for the primed reaction of fraction III. The product formed by the unprimed reaction was a glucan containing principally α-1,4 linkages with some α-1,6 linkages. The primer, maltose, at a concentration of 0.5m inhibited the synthesis of the unprimed product./p
机译：通过DEAE-纤维素塔从菠菜叶获得多种形式的ADP-葡萄糖-α-1,4-葡聚糖α-4-葡糖糖基转移酶。在高浓度的一些盐和牛血清白蛋白的存在下，在从塔中洗脱的四个级分的一个（转凝凝集溶胶酶III）中发现未提生的活性。除了具有未提生的活性之外，转甘溶胶酶III对于ADP-葡萄糖，具有较低的 k m ，更高的 k m 对于牡蛎糖原，对麦芽糖，麦芽糖和淀粉蛋白β极见β-极限糊精比级分I，II和IV更高的热敏感性和较低的亲和力。此外，低浓度的直链淀粉，支链淀粉和兔肝糖糖苷的动力学是晶颗粒酶III的非线性。转胶酶I，II和IV的性质通常彼此相似。在地生理浓度的ADP-葡萄糖的未预期反应的速率大于少数馏分III的引发反应的反应。由未提生的反应形成的产物是含有主要α-1,4连杆的葡聚糖，其具有一些α-1,6键。浓度为0.5m的底漆，麦芽糖抑制了未预期产品的合成。

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