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The formation of sulphur-containing amino acids in germinating seeds of rape (Brassica napus L.)

机译:在发芽的油菜籽中形成含硫氨基酸(芸苔Napus L.)

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pSodium [sup35/supS]sulphide was fed to batches of germinating rapeseed, in some instances with the addition of unlabelled cysteine. Both the total radioactivity and specific radioactivity of the free sulphur-containing amino acids were examined. Cysteine and homocysteine were rapidly labelled; label subsequently appeared in cystathionine and methionine. The results obtained indicated that both the sulphydration and trans-sulphuration pathways were operating. This conclusion was reinforced by the results of experiments in which batches of rapeseed were incubated with l-[sup14/supC]homoserine. These showed the formation of labelled homocysteine, cystathione and methionine. It was thought the trans-sulphuration pathway was making the greater contribution to the biosynthesis of methionine in germinating rapeseed./p
机译:在一些情况下,将>钠[ 35℃> s]硫化物批量生种发芽油菜籽,其中包含未标记的半胱氨酸。检查无含硫含氨基酸的总放射性和特异性放射性。半胱氨酸和同型半胱氨酸迅速标记;标签随后出现在胱硫胺和甲硫氨酸中。得到的结果表明,硫酸和反式硫化途径都是操作。通过实验结果加强了该结论,其中将批次的油菜与L - [ 14℃] Homeserine一起温育。这些显示形成标记的同型半胱氨酸,胱硫脲和蛋氨酸。据认为,转硫化途径对发芽油菜籽的蛋氨酸生物合成产生更大的贡献。

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