首页> 外文期刊>Journal of Virology >Filamentous Bacterial Viruses VII. Inhibition of fd Deoxyribonucleic Acid Synthesis After a Temperature Jump into Protein-Synthesis Inhibitors
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Filamentous Bacterial Viruses VII. Inhibition of fd Deoxyribonucleic Acid Synthesis After a Temperature Jump into Protein-Synthesis Inhibitors

机译:丝状细菌病毒VII。温度跳跃到蛋白质合成抑制剂后抑制FD脱氧核糖核酸合成

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Synthesis of fd deoxyribonucleic acid (DNA) was stopped by transferring infected bacteria from 32 C into chloramphenicol or serine hydroxamate at 42 C, but not by addition of these antibiotics at 32 C, and not by a temperature change in the absence of antibiotics. The inhibition of fd DNA synthesis by serine hydroxamate at 42 C was reversed by excess serine. The ability to synthesize fd DNA at 42 C in chloramphenicol was rescued by delaying the addition of chloramphenicol for a few minutes after the transfer from 32 to 42 C. The colony-forming ability of abortively infected bacteria was also rescued from “killing” by delaying the addition of chloramphenicol after a transfer from 32 to 42 C.
机译:通过在42℃下将感染的细菌从32℃转移到氯霉素或丝氨酸羟肟酸盐中,终止于32℃,而不是通过在32℃下加入这些抗生素,而不是通过抗生素的温度变化来终止FD脱氧核糖核糖核酸(DNA)。通过过量的丝氨酸在42℃下抑制FD DNA合成的丝氨酸羟肟酸酯。通过在从32-22℃转移后延迟加入氯霉素在氯霉素中延迟加入氯霉素在42℃下抵抗氯霉素在42℃下合成FD DNA的能力。通过延迟,血液形成感染细菌的菌落形成能力也被扣除了“杀死”在32至42℃转移后加入氯霉素。

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