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首页> 外文期刊>Journal of Virology >Structure, subunit composition, and molecular weight of RD-114 RNA.
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Structure, subunit composition, and molecular weight of RD-114 RNA.

机译：结构，亚基组合物和RD-114 RNA的分子量。

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The properties and subunit composition of the RNA extracted from RD-114 virions have been studied. The RNA extracted from the virion has a sedimentation coefficient of 52S in a nondenaturing aqueous electrolyte. The estimated molecular weight by sedimentation in nondenaturing and weakly denaturing media is in the range 5.7 X 10(6) to 7.0 X 10(6). By electron microscopy, under moderately denaturing conditions, the 52S molecule is seen to be an extended single strand with a contour length of about 4.0 mum corresponding to a molecular weight of 5.74 X 10(6). It contains two characteristic secondary structure features: (i) a central Y- or T-shaped structure (the rabbit ears) with a molecular weight of 0.3 X 10(6), (ii) two symmetreically disposed loops on each side of and at equal distance from the center. The 52S molecule consists of two half-size molecules, with molecular weight 2.8 X 10(6), joined together within the central rabbit ears feature. Melting of the rabbit ears with concomitant dissociation of the 52S molecule into subunits, has been caused by either one of two strongly denaturing treatments: incubation in a mixture of CH3HgOH and glyoxal at room temperature, or thermal dissociation in a urea-formamide solvent. When half-size molecules are quenched from denaturing temperatures, a new off-center secondary structure feature termed the branch-like structure is seen. The dissociation behavior of the 52S complex and the molecular weight of the subunits have been confirmed by gel electrophoresis studies. The loop structures melt at fairly low temperatures; the dissociation of the 52S molecule into its two subunits occurs at a higher temperature corresponding to a base composition of about 63% guanosine plus cytosine. Polyadenylic acid mapping by electron microscopy shows that the 52S molecule contains two polyadenylic acid segments, one at each end. It thus appears that 52S RD-114 RNA consists of two 2.8 X 10(6) dalton subunits, each with a characteristic secondary structure loop, and joined at the 5' ends to form the rabbit ears secondary structure feature. The observations are consistent with but do not require the conclusion that the two 2.8 X 10(6) dalton subunits of 52S RD-114 RNA are identical.
机译：研究了从RD-114病毒藻中提取的RNA的性质和亚基组成。从Viriv中提取的RNA具有沉降系数为52s，其在非生种水性电解质中。通过NondeNativing和弱变性介质中沉淀的估计的分子量为5.7×10（6）至7.0×10（6）。通过电子显微镜检查，在适度的变性条件下，52S分子被观察到延伸的单链，其轮廓长度为约4.0毫米，对应于5.74×10（6）的分子量。它含有两个特征的二级结构特征：（i）一种分子量为0.3×10（6），（ii）两侧和处的两种对称的对称的y型或T形结构（兔耳），（II）距离中心的距离相同。 52s分子由两个半尺寸的分子组成，分子量为2.8×10（6），在中央兔耳朵特征内连接在一起。兔耳朵的含量伴随着52s分子分解成亚基，这是由两个强烈变性治疗中的一种引起的：在室温下在CH3HGoH和乙二醛的混合物中孵育，或在尿素 - 甲酰胺溶剂中热解离。当从变性温度淬火半尺寸分子时，可以看到新的偏心二次结构特征被称为分支结构。通过凝胶电泳研究证实了52s复合物的解离行为和亚基的分子量。环结构在相当低的温度下熔化;将52s分子的解离在其两个亚基的较高温度下发生在对应于约63％鸟氨酸加胞嘧啶的基础组合物的较高温度下。通过电子显微镜的聚腺苷酸映射表明，52s分子含有两个聚腺苷酸区段，每根含有一个。因此，似乎52S RD-114 RNA由两个2.8×10（6）个DALTON亚基组成，每个亚单位具有特征二次结构回路，并在5'端连接以形成兔耳的二级结构特征。观察结果与但不需要的结论是52s RD-114 RNA的22.8×10（6）道尔顿亚基是相同的。

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