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首页> 外文期刊>Journal of Virology >Physical properties of moloney murine leukemia virus high-molecular-weight RNA: a two subunit structure.
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Physical properties of moloney murine leukemia virus high-molecular-weight RNA: a two subunit structure.

机译：莫尼鼠白血病病毒高分子量RNA的物理性质：两个亚基结构。

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The high-molecular-weight RNA of Moloney murine leukemia virus (MuLV) was analyzed by sedimentation equilibrium ultracentrifugation. Molecular weights of 7.2 x 10(6) and 3.4 x 10(6) were found for the native and subunit forms, respectively, indicating that the native structure is a dimer. S20,w and frictional coefficients were determined for MuLV RNA by analytical velocity centrifugation as a function of ionic strength. The apparent S20,w of native MuLV RNA was 47.3, 57.4, and 66.5 in 0.01, 0.1, and 0.20 M Na+, respectively; the corresponding frictional coefficients were 5.44, 4.48, and 3.87. Native RNA was estimated by circular dichroism to be 85% helical, whereas denatured RNA was 54% helical. Thermal denaturation profiles were obtained from uv absorbance scans. Melting temperatures of 57 and 68 C were obtained for high-molecular-weight RNA in 0.01 M Na+ and 0.122 M Na+, 1mM Mg2+, respectively. van't Hoff plots of the thermal denaturation data gave enthalpies for the helix-coil transition of 21,600 cal (ca. 90,500 J) per mol of cooperatively melting unit in high salt and 19,600 cal (ca. 82,100 J) per mol in low salt, consistent with both base stacking and pairing. The melting of Mu LV RNA occurred over a broad temprange and van't Hoff plots were linear over most of the melting range, indicating a noncooperative process of helix stabilization.
机译：通过沉淀平衡超速离心分析莫尼鼠白血病病毒（MULV）的高分子量RNA。分子量为7.2×10（6）和3.4×10（6），分别用于天然和亚基形式，表明天然结构是二聚体。通过作为离子强度的函数，通过分析速度离心确定Mulv RNA的S20，W和摩擦系数。含有0.01,0.1和0.20m Na +的天然Mulv RNA的表观S20，W的天然Mulv RNA为47.3,57.4和66.5;相应的摩擦系数为5.44,4.48和3.87。通过圆形二色性呈循环螺旋估计本地RNA，而变性的RNA为54％螺旋。从UV吸光度扫描获得热变性曲线。为0.01M Na +和0.122m Na +，1mm Mg2 +的高分子量RNA获得57和68℃的熔化温度。 Vane Not Vuff Denation数据的展位局部为螺旋线圈过渡的焓为每摩尔在低盐中每摩尔和19,600克（CA.82,100 J）的合作熔炼单元的21,600克（CA.90,500 J）。 ，与基础堆叠和配对一致。 Mu LV RNA的熔化发生在宽的温度范围内，并且Van'tHoff图在大部分熔化范围内是线性的，表明螺旋稳定性的非自由作用过程。

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