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首页> 外文期刊>Journal of Virology >Simian virus 40-specific proteins in HeLa cells infected with nondefective adenovirus 2-simian virus 40 hybrid viruses.
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Simian virus 40-specific proteins in HeLa cells infected with nondefective adenovirus 2-simian virus 40 hybrid viruses.

机译：Simian病毒40种特异性蛋白质在HeLa细胞中感染了NondFective腺病毒2-猿猴病毒40杂种病毒。

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摘要

The synthesis of simian virus 40 (SV40)-specific proteins in HeLa cells infected with the nondefective adenovirus 2 (Ad2)-SV40 hybrid viruses, Ad2+ND2, Ad2+ND3, Ad2+ND4, and Ad2+ND5, was investigated. Infected-cell proteins were labeled with radioactive amino acids late after infection, when host protein synthesis was shut off, and analyzed by polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulfate. All polypeptides normally seen in Ad2-infected cells were found in cells infected by the hybrid viruses. In addition to the Ad2-specific proteins, cells infected with Ad2+ND2 contain two SV40-specific proteins with apparent molecular weights of 42,000 and 56,000, cells infected with Ad2+ND4 contain one protein with an apparent molecular weight of 56,000, and cells infected with Ad2+ND5 contain one protein with an apparent molecular weight of 42,000. Cells infected with Ad2+ND3 do not contain detectable amounts of proteins not seen during Ad2 infection. Pulse-chase experiments demonstrate that the SV40-specific proteins induced by Ad2+ND2, Ad2+ND4, and Ad2+ND5 are metabolically unstable. These proteins are not present in purified virions. Two nonstructural Ad2-specific proteins have been demonstrated in Ad2 and hybrid virus-infected cells which have a smaller apparent molecular weight after a short pulse than after a pulse followed by a chase. The molecular weight increase during the chase may be caused by the addition of carbohydrate to a polypeptide backbone.
机译：研究了用Nondefective Adenovirus 2（Ad2）-Sv40杂交病毒，Ad2 + Nd2，Ad2 + Nd3，Ad2 + Nd4和Ad2 + Nd5感染的HeLa细胞中Simian病毒40（SV40）的合成蛋白质。感染后晚期用放射性氨基酸标记感染细胞蛋白，当宿主蛋白质合成被关闭时，并通过聚丙烯酰胺凝胶电泳在十二烷基硫酸钠存在下进行分析。在受杂交病毒感染的细胞中发现通常在Ad2感染细胞中看到的所有多肽。除了AD2特异性蛋白外，用Ad2 + Nd2感染的细胞含有两个具有42,000和56,000的表观分子量的SV40特异性蛋白质，所述细胞感染Ad2 + Nd4含有一种表观分子量为56,000的蛋白质和感染细胞Ad2 + Nd5含有一种表观分子量为42,000的蛋白质。感染AD2 + ND3的细胞不含在AD2感染期间未见的可检测量的蛋白质。脉冲序列实验表明，由Ad2 + Nd2，Ad2 + Nd4和Ad2 + Nd5诱导的SV40特异性蛋白质是代谢不稳定的。这些蛋白质不存在于纯化的病毒中。在Ad2和杂合病毒感染细胞中已经证明了两种非结构AD2特异性蛋白质，其在短脉冲之后的表观分子量小于脉冲之后的较小的脉冲，其次被追逐。追逐期间的分子量增加可能是由向多肽骨架中加入碳水化合物引起的。

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    《Journal of Virology》 |1975年第5期|共12页
  • 作者

    G Walter; H Martin;

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  • 收录信息 美国《科学引文索引》(SCI);
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