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首页> 外文期刊>Journal of Virology >N-Glycosidase activity in extracts of Bacillus subtilis and its inhibition after infection with bacteriophage PBS2.
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N-Glycosidase activity in extracts of Bacillus subtilis and its inhibition after infection with bacteriophage PBS2.

机译：枯草芽孢杆菌提取物中的N-糖苷酶活性及其在噬菌体PBS2感染后的抑制作用。

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摘要

We have detected in crude extracts of Bacillus subtilis an N-glycosidase activity which catalyzes the release of free uracil from DNA of the subtilis phage PBS2 labeled with [3H]uridine. This DNA contains deoxyuridine instead of thymidine. The enzyme is active in the presence of 1.0 mM EDTA and under these conditions Escherichia coli or T7 DNA labeled with [3H]thymidine is not degraded to labeled acid-soluble products. The activity resembles an N-glycosidase from E. coli which releases free uracil from DNA containing deaminated cytosine residues. Both enzymes in crude extracts are active in the presence of EDTA, do not require dialyzable co-factors, and have the same pH optimum. They differ in that the enzyme from E. coli is more sensitive to heat, sulfhydryl reagents, and salt. The enzyme from B. subtilis is inactive on DNA containing 5-bromouracil or hydroxymethyluracil. Extracts of PBS2-infected B. subtilis lose the N-glycosidase activity within 4 min after infection and contain a factor that inhibits the N-glycosidase activity within 4 min after infection and contain a factor that inhibits the N-glycosidase activity in extracts of uninfected cells in vitro.
机译：我们已经检测到枯草芽孢杆菌的粗提物中的N-糖苷酶活性，其催化从枯草芽孢杆菌噬菌体PBS2的DNA的游离尿嘧啶的释放释放，所述枯草芽孢杆菌噬菌体PBS2与尿苷标记的。该DNA含有脱氧尿苷代替胸苷。酶在1.0mM EDTA存在下活跃，并且在这些条件下，用[3H]胸苷标记的大肠杆菌或T7 DNA不会降解到标记的酸溶解产物中。该活性类似于来自大肠杆菌的N-糖苷酶，其从含有脱胺胞嘧啶残基的DNA释放自由尿嘧啶。在粗提取物中的两种酶都在EDTA存在下活跃，不需要透析的共同因子，并且具有相同的pH值。它们的不同之处在于，来自大肠杆菌的酶对热，巯基试剂和盐更敏感。来自B.枯草芽孢杆菌的酶在含有5-溴脲或羟甲基甲基脲的DNA上无活性。 PBS2感染的B.枯草芽孢杆菌在感染后4分钟内丧失N-醇酶活性，含有在感染后4分钟内抑制N-醇酶活性的因素，并含有抑制未染色的提取物中的N-醇酶活性的因素细胞体外。

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