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首页> 外文期刊>Journal of Virology >Host-dependent restriction of mengovirus replication. II. Effect of host restriction on late viral RNA synthesis and viral maturation.
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Host-dependent restriction of mengovirus replication. II. Effect of host restriction on late viral RNA synthesis and viral maturation.

机译：脊髓病毒复制的宿主依赖性限制。 II。宿主限制对晚病毒RNA合成和病毒成熟的影响。

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Restricted mengovirus replication in Mandin-Darby bovine kidney (MDBK) cells is characterized by a 400-fold reduction in infectious virus yield and a 40-fold increase in the production of noninfectious virus. Using conditions which insure that all MDBK cells are infected, virus-specific RNA and protein synthesis were measured in the restrictive host and in a permissive host for mengovirus, HeLa cells. Labeling kinetics and sucrose gradient analysis of mengovirus-specific RNA from MDBK cells show a reduction of 10-fold in virion RNA, 5-fold in double-stranded RNA, and 12.5-fold in single-stranded RNA. The viral RNA biosynthetic processes which occur late in the replicative cycle and result in the production of 90% of the single-stranded viral RNA that is packaged into capsid proteins in the permissive host are absent in restrictive MDBK cells. Viral protein synthesis as measured by labeled viral-specific polysome is decreased, and there is an accumulation of 80S subviral particles in the restricted host. It is suggested that restriction may act at a number of stages of viral replication and maturation.
机译：在Mandin-Darby牛肾（MDBK）细胞中受限制的复制可治疗的特征在于在感染性病毒的产量400倍的减少和生产非感染性病毒的40倍的增加。使用确保所有MDBK细胞感染的条件，在限制宿主中测量病毒特异性RNA和蛋白质合成，并在Mengovirus，Heenovirus，Heara细胞中测量。标记动力学和从MDBK细胞可治疗特异性RNA蔗糖梯度分析表明在病毒粒子RNA的减少的10倍，在双链RNA的5倍，并在单链RNA 12.5倍。在重复循环中发生晚期的病毒RNA生物合成过程并导致在允许宿主中包装成杂种蛋白的90％的单链病毒RNA的生产中不存在于限制性的MDBK细胞中。通过标记的病毒特异性多肌组测量的病毒蛋白合成减少，限制宿主中的80s子病毒颗粒的积累。建议限制可以在一些病毒复制和成熟的阶段起作用。

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