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首页> 外文期刊>Journal of Virology >Levels of Rat Cellular RNA Homologous to Either Kirsten Sarcoma Virus or Rat Type-C Virus in Cell Lines Derived from Osborne-Mendel Rats
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Levels of Rat Cellular RNA Homologous to Either Kirsten Sarcoma Virus or Rat Type-C Virus in Cell Lines Derived from Osborne-Mendel Rats

机译：对奥斯本孟德尔大鼠的细胞系中Kirsten Sarcoma病毒或大鼠C型病毒同源的大鼠细胞RNA水平

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DNA transcripts from V-NRK and RT21c rat type-C viruses were found to differ in their sequence homology to Kirsten and Harvey sarcoma viruses. V-NRK DNA transcripts consistently had homology to Kirsten and Harvey sarcoma virus, whereas RT21c DNA transcripts did not. To explain the differences, the nucleic acids and structural proteins of the two type-C viruses, released from each of two cell lines derived from Osborne-Mendel rats, were analyzed by molecular hybridization and competition radioimmunoassays. The p30 and p12 structural proteins of the two viruses were found to be highly related immunologically. In the V-NRK virus preparation, two sets of distinct RNA sequences were found in approximately equal amounts. One set is homologous to Ki-SV, and the other homologous to RT21c. In contrast, the RT21c virus preparation was found to contain a different ratio of these sequences. In this case the RT21c-like RNA sequences are present in 100-fold excess as compared to the additional Ki-SV specific sequences. Both NRK and RT21c cells contain in their DNA the full complement of Ki-SV homologous sequences, but NRK cells express much higher levels of these Ki-SV sequences in their RNA. These additional sequences, not homologous to RT21c, which are detected in uninfected NRK cellular RNA or V-NRK rat virus, could also be detected in the 60-70S RNA from a Moloney mouse type-C virus released from the NRK cells infected with the Moloney type-C virus. The results suggest that type-C viruses released from NRK cells incorporate species of RNA present in NRK cells which are homologous to Kirsten and Harvey sarcoma viruses. Either these sequences are of cellular origin, or rat cells contain two endogenous viruses with completely distinct nucleic acid sequences.
机译：来自V-NRK和RT21C大鼠C型病毒的DNA转录物在其对Kirsten和Harvey Sarcoma病毒的序列同源物中不同。 V-NRK DNA转录物一直对Kirsten和Harvey Sarcoma病毒有同源性，而RT21C DNA转录物没有。为了解释从源自奥斯本 - 孟德尔大鼠衍生的两种细胞系中释放的两种类型C型病毒的差异，分子杂交和竞争放射线测量分析。发现两种病毒的P30和P12结构蛋白在免疫上具有高度相关的免疫。在V-NRK病毒制剂中，在大致相等的量中发现两组不同的RNA序列。一组与Ki-Sv和其他与RT21C同源同源物质。相反，发现RT21C病毒制剂含有不同的这些序列的比例。在这种情况下，与附加的Ki-SV特异性序列相比，RT21C样RNA序列存在于100倍过量中。 NRK和RT21C细胞均含有其DNA的全部补充Ki-SV同源序列，但NRK细胞在其RNA中表达了更高水平的这些Ki-SV序列。这些附加序列，与RT21C的其他序列，其在未感染的NRK细胞RNA或V-NRK大鼠病毒中也可以在60-70S RNA中检测来自从感染的NRK细胞释放的莫尼小鼠类型-C病毒中莫洛尼型病毒。结果表明，从NRK细胞中释放的C-C病毒包含与Kirsten和Harvey Sarcoma病毒同源的NRK细胞中存在的RNA。这些序列是细胞来源的，或大鼠细胞含有两个具有完全不同的核酸序列的内源病毒。

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