首页> 外文期刊>Journal of Virology >Excision of Thymine Dimers In Vitro by Extracts of Bacteriophage-Infected Escherichia coli
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Excision of Thymine Dimers In Vitro by Extracts of Bacteriophage-Infected Escherichia coli

机译:通过噬菌体感染大肠杆菌提取物在体外切除胸腺嘧啶二聚体

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Extracts of DNA polymerase I defective Escherichia coli infected with phage T4 contain an exonuclease activity that removes thymine dimers from UV-irradiated DNA previously nicked with T4 UV endonuclease. This activity is not expressed if cells are infected in the presence of chloramphenicol. The enzyme has a requirement for divalent cation and is not affected by caffeine, but excision is inhibited in the presence of proflavine. The enzyme is present in all phage T4 mutants thus far examined, including 25 UV-sensitive mutants isolated during the course of the experiments, all of which are defective in the v gene. A similar activity can be detected in cells infected with phages T2, T3, and T6, but not in cells infected with phage T7.
机译:DNA聚合酶I的提取物有缺陷的大肠杆菌感染的噬菌体T4含有外切核酸酶活性,从预先用T4 UV内切核酸酶中取出来自UV辐照的DNA的胸腺嘧啶二元。如果在氯霉素存在下感染细胞,则不表达该活性。酶具有二价阳离子的要求,并且不受咖啡因的影响,但在预氟灵线存在下抑制切除。酶存在于迄今为止的所有噬菌体T4突变体中,包括在实验过程中分离的25个紫外敏感突变体,所有这些突变体在血液中分离,所有这些都在 v 基因中有缺陷。可以在用噬菌体T2,T3和T6感染的细胞中检测类似的活性,但不在用噬菌体T7感染细胞中。

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