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首页> 外文期刊>Journal of Virology >Interactions of Polyoma and Mouse DNAs I. Lytic Infection of Bromodeoxyuridine-Prelabeled Mouse Embryo Cells
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Interactions of Polyoma and Mouse DNAs I. Lytic Infection of Bromodeoxyuridine-Prelabeled Mouse Embryo Cells

机译：多元瘤和小鼠DNAs的相互作用I.溴脱氧亚氨氨酸预先标记小鼠胚胎细胞的裂解感染

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On CsCl isopycnic centrifugation of the DNA extracted from secondary mouse embryo (ME) cultures grown in the presence of 5-bromodeoxyuridine (BUdR) and 5-fluorodeoxyuridine (FUdR) for 40 h, 10 to 25% of the DNA was found to be unsubstituted, 70 to 80% was bromouracil-hybrid DNA, and 5 to 10% was heavy DNA. These results together with cell number determinations, autoradiography, and Feulgen microspectrophotometry revealed three types of cells in these cultures: (i) 60 to 80% of the cells replicated their DNA once, divided, and then stopped mitotic activity, (ii) 5 to 10% were going through a second round of DNA replication; whereas (iii) 10 to 30% did not replicate DNA during the BUdR-FUdR exposure. After the transfer of these cultures to normal medium (without BUdR-FUdR), up to 20% of the cells resumed DNA synthesis asynchronously within 60 h, but no increase in cell number was observed. BUdR-FUdR-treated cultures, which were infected with polyoma virus in the absence of the thymidine analogues, supported a lytic infection to the same extent as did untreated ME cultures. This was concluded from the similar number of cells, which were induced to synthesize DNA, from the similar replication rate of the viral DNA, from the similar number of cells containing polyoma capsid proteins, and from the similar yields of progeny virus determined by hemagglutination and plaque formation. Thus, BUdR-prelabeled ME cultures are suitable for the investigation of interactions of the polyoma and mouse genomes during the lytic infection.
机译：在CSCL等二三基于在5-溴酰基嘌呤（Budr）和5-氟吲哚脲（Fudr）存在下生长的二次小鼠胚胎（ME）培养物中的DNA对40小时，发现10-25％的DNA被发现是未取代的，70至80％是溴尿嘧啶杂交DNA，5至10％是重的DNA。这些结果与细胞数判定，放射造影和Feulgen微痉挛中揭示了这些培养物中的三种类型的细胞：（i）60至80％的细胞将其DNA复制一次，分开，然后停止有丝分裂活性，（ii）5至10％正在经过第二轮DNA复制;然而（iii）10至30％在Budr-Fudr暴露期间没有复制DNA。在将这些培养物转移到正常培养基（没有BudR-Fudr）后，高达20％的细胞在60h内异步恢复了DNA合成，但观察到细胞数量没有增加。在没有胸苷类似物的情况下感染多瘤病毒的Budr-Fudr治疗培养物，在相同程度的情况下支持裂解感染，就像未经处理的我的培养物一样。从类似的细胞中得出结论，从含有多瘤衣壳蛋白的相似的细胞数量，从病毒DNA的类似的细胞中诱导合成DNA的细胞，以及通过血凝和血凝率测定的后代病毒的相似产量斑块形成。因此，Budr-PreLabeled ME培养物适用于在裂解感染期间对多元瘤和小鼠基因组的相互作用的研究。

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    《Journal of Virology》 |1974年第2期|共9页
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    Hans Türler;

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  • 收录信息 美国《科学引文索引》(SCI);
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