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首页> 外文期刊>Journal of Virology >Protein Synthesis in Newcastle Disease Virus-Infected Chicken Embryo Cells
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Protein Synthesis in Newcastle Disease Virus-Infected Chicken Embryo Cells

机译：蛋白质合成在新城疫病毒感染的鸡胚细胞

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A double-isotopic label difference analysis of polyacrylamide gels has been used to distinguish between cellular and viral protein accumulation in infected cells and to quantify the kinetics of accumulation of viral polypeptides. This technique, coupled with the determination of total radioactive amino acid incorporation in infected cultures, has revealed the following kinetic patterns. Viral polypeptides are first detected in infected cultures 2.0 to 2.5 h postinfection. The rate of accumulation of radioactive amino acids in viral polypeptides increases to a maximum (30 to 35% of the rate of accumulation in uninfected control cultures), whereas the rate of accumulation of radioactive amino acids in host-cell protein decreases to a minimum (20% of the rate of accumulation in uninfected control cultures) by 5 to 6 h postinfection. All of the viral polypeptides detected late in infection are also present at the earlier times, and the major virion structural polypeptides are present in approximately the same (N/G-2, 53K) or slightly increasing (L, G-1, M) relative amounts. One peak area containing a nonstructural glycopeptide with an apparent molecular weight of 66,000 shows significant alterations in rates of accumulation during infection. Inhibition in the rate of radioactive amino acid incorporation into both trichloroacetic acid-soluble and acid-precipitable material during infection has been demonstrated. However, these two inhibition phenomena can be uncoupled temporally by incubating infected cultures at 36 C instead of the usual 40 C, suggesting that they may not be directly related.
机译：聚丙烯酰胺凝胶的双同位素标记差分分析已被用于区分感染细胞中的细胞和病毒蛋白质积累，并量化病毒多肽的积累动力学。该技术与在感染培养物中的总放射性氨基酸掺入时，揭示了以下动力学图案。首先在感染的培养物中检测到病毒多肽2.0至2.5小时。病毒多肽中放射性氨基酸的积累速率增加到最大值（无感染的对照培养物中积累速率的30％至35％），而宿主细胞蛋白质中放射性氨基酸的积累速率降至最低（未感染的对照培养物的积累速度的20％）出现5至6小时。在早期感染后检测到的所有病毒多肽也存在于早期的时间，并且主要的病毒赛结构多肽以大致相同（n / g-2,53k）或略微增加（l，g-1，m）。相对金额。含有非结构糖肽的一个峰面积，表观分子量为66,000显示出感染期间积累率的显着改变。已经证明了在感染期间掺入三氯乙酸可溶性和酸性易燃材料中的放射性氨基酸率掺入的抑制作用。然而，这两种抑制现象可以通过在36℃下孵育感染的培养物而不是通常的40℃来在时间上脱节，这表明它们可能不会直接相关。

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