Marek's Disease Herpesvirus-Induced DNA Polymerase

John A. Boezi; Lucy F. Lee; Robert W. Blakesley; Mark Koenig; Howard C. Towle

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Marek's Disease Herpesvirus-Induced DNA Polymerase

机译：Marek的疾病疱疹病毒诱导的DNA聚合酶

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Infection of duck embryo fibroblasts by Marek's disease herpesvirus (MDHV), strain GA, led to the induction of a novel DNA polymerase. This novel DNA polymerase, designated MDHV-induced DNA polymerase, could be distinguished from the DNA polymerase activities of uninfected duck embryo fibroblasts by its chromatographic behavior on phosphocellulose, by its sedimentation coefficient, and by its catalytic properties. The characteristics of MDHV-induced DNA polymerase which had been purified by phosphocellulose chromatography were investigated. The sedimentation coefficient of the enzyme, as determined by sucrose density gradient centrifugation in the presence of 0.25 M KCl, was 5.9S. From this sedimentation coefficient, the molecular weight of MDHV-induced DNA polymerase was estimated to be 100,000. MDHV-induced DNA polymerase could not effectively use either poly(dA)·oligo(dT)_12-18 or poly(dC)·oligo(dG)_12-18 as a template-primer. The DNA polymerases from uninfected duck embryo fibroblasts could use these synthetic template-primers. MDHV-induced DNA polymerase also could not use poly(rA)·oligo(dT)_12-18 or poly(rC)·oligo(dG)_12-18 as template-primers or oligo(dT)_12-18 as a primer, indicating that it was not a polymerase of the type R-DNA polymerase, reverse transcriptase, or a terminal nucleotidyl transferase. In vitro synthesis of DNA by MDHV-induced DNA polymerase was markedly inhibited by the addition of (NH₄)₂SO₄ to the reaction mixture.

机译：Marek疾病疱疹病毒（MDHV），应变Ga感染鸭胚胎成纤维细胞，导致了一种新型DNA聚合酶的诱导。该新型DNA聚合酶，指定的MDHV诱导的DNA聚合酶，可以通过其沉积系数的磷酸纤维素的色谱行为和其催化性能来区分除染鸭胚胎成纤维细胞的DNA聚合酶活性。研究了通过磷纤维素色谱纯化的MDHV诱导的DNA聚合酶的特性。通过在0.25M KCl存在下通过蔗糖密度梯度离心确定的酶的沉降系数为5.9 S 。从这种沉降系数来看，MDHV诱导的DNA聚合酶的分子量估计为100,000。 MDHV诱导的DNA聚合酶不能有效地使用聚（DA）·寡（DT）<亚> 12-18 或聚（DC）·寡（DG）_{12-18 模板底漆。来自无感染的鸭胚成纤维细胞的DNA聚合酶可以使用这些合成模板引物。 MDHV诱导的DNA聚合酶也不能使用聚（Ra）·寡核苷酸（DT）_{12-18 或聚（RC）·寡（DG）_{12-18 作为模板 - 作为底漆的预期或寡聚（DT）_{12-18 ，表明它不是R-DNA聚合酶，逆转录酶或末端核苷酸转移酶的聚合酶。通过加入（NH _{4 ）_{2-4 通过加入（NH _{4次>） 4-so>抑制DNA的体外合成DNA。。}}}}}}} 展开▼

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《Journal of Virology》 |1974年第5期|共11页

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John A. Boezi; Lucy F. Lee; Robert W. Blakesley; Mark Koenig; Howard C. Towle;
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收录信息美国《科学引文索引》(SCI);

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专利

1. Marek's Disease Herpesvirus-Induced DNA Polymerase [J] . John A. Boezi, Lucy F. Lee, Robert W. Blakesley, Journal of Virology . 1974,第5期

机译：Marek的疾病疱疹病毒诱导的DNA聚合酶

2. Marek's Disease Herpesvirus-Induced DNA Polymerase [J] . John A. Boezi, Lucy F. Lee, Robert W. Blakesley, Journal of Virology . 1974,第5期

机译：Marek的疾病疱疹病毒诱导的DNA聚合酶

3. Effects of T-2 Toxin on Turkey Herpesvirus-Induced Vaccinal Immunity Against Marek's Disease [J] . Kufuor-Mensah E., Reed W. M., Sleight S., Avian Diseases . 2016,第1期

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4. Cell-free incorporation of photo-crosslinkable p-benzoyl-L-phenylalanine into the polymerase subunits α and ε of Escherichia coli DNA polymerase III [C] . Kiyoshi Ozawa, Gottfried Otting, Nicholas E. Dixon Incorporating of the Australian Society for Biochemistry and Molecular Biology Combio . 2009

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5. Recombinant DNA molecules and herpesviruses as potential immunogens against infectious bursal disease and Marek's disease viruses. [D] . Hulse, Diane Jaree. 2002

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6. Mareks Disease Herpesvirus-Induced DNA Polymerase [O] . John A. Boezi, Lucy F. Lee, Robert W. Blakesley, 1974

机译：马立克氏病疱疹病毒诱导的DNA聚合酶

7. Identification of the Neoplastically Transformed Cells in Marek's Disease Herpesvirus-Induced Lymphomas: Recognition by the Monoclonal Antibody AV37 [O] . Burgess, Shane C., Davison, T. Fred 2002

机译：马立克氏病疱疹病毒诱导的淋巴瘤中肿瘤转化细胞的鉴定：单克隆抗体AV37的识别。

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