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首页> 外文期刊>Journal of Virology >Gene Expression During the Development of Bacteriophage Φ29 III. Analysis of Viral-Specific Protein Synthesis with Suppressible Mutants
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Gene Expression During the Development of Bacteriophage Φ29 III. Analysis of Viral-Specific Protein Synthesis with Suppressible Mutants

机译：噬菌体φ29III发育过程中的基因表达。抑制突变体的病毒特异性蛋白质合成分析

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摘要

Fifty-four suppressible mutants of bacteriophage Φ29 have been isolated with a variety of mutagens and assigned to eight complementation groups. Viral-specific protein synthesis in UV light-irradiated, nonsuppressing Bacillus subtilis 60084 was analyzed with exponential acrylamide gels. Four additional Φ29 proteins which were undetected on ordinary acrylamide gels are reported in this paper. Five phage Φ29 proteins have been unambiguously assigned to specific cistrons. Two cistrons had pleiotropic effects on viral protein synthesis. Mutants in cistrons I or II were unable to synthesize DNA in nonsuppressing bacteria. Mutants in cistron I were unable to attach viral chromosomes to the host cell membrane, and the protein responsible for this function has been identified. The other viral protein playing a role in phage Φ29 DNA synthesis is also identified and assigned to cistron II. Mutants in cistron II can attach viral chromosomes to membrane, but cannot synthesize DNA in nonsuppressing bacteria.
机译：噬菌体φ29的五十四个抑制突变体已被各种诱变组分离，并分配到八个互补组。用指数丙烯酰胺凝胶分析了UV光辐照的病毒特异性蛋白质合成，非丙烯酰胺凝胶。本文报道了四种未检测到普通丙烯酰胺凝胶上的φ29蛋白。五个噬菌体φ29蛋白已明确地分配给特定的排名。两个轮柱对病毒蛋白合成具有抗脂肪效应。传感器I或II中的突变体无法在非抑制细菌中合成DNA。 Cistron I中的突变体无法将病毒染色体附着到宿主细胞膜，并且已经鉴定了对该功能的负责的蛋白质。在φ29DNA合成中发挥作用的其他病毒蛋白也被鉴定并分配给Cistron II。 Cistron II中的突变体可以将病毒染色体附着到膜，但不能在非抑制细菌中合成DNA。

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