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首页> 外文期刊>Journal of Virology >Intracellular Virus-Specific Structures and RNAs in Oncornavirus-Producing Human Cells
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Intracellular Virus-Specific Structures and RNAs in Oncornavirus-Producing Human Cells

机译:在oncornavirus的人体细胞中细胞内病毒特异性结构和RNA

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Two kinds of virus-specific structures were isolated from the cytoplasm of Detroit-6 and human amnion cells producing oncornavirus-like particles. These structures represented A particles with the diameter of 70 to 80 nm and aggregated strands of nucleocapsids with the diameter of 3 and 6 nm. The structures were separated from cellular contaminants by isopycnic banding in linear sucrose gradients and subsequently further purified by sedimentation in velocity sucrose gradients. Their sedimentation coefficient was 250 and 150S, respectively. Both structures contain 60, 45, and 35S RNA species, and 150S structures also contained 20S RNA. The 35 and 20S RNA from the 150S structure formed hybrids with DNA enzymatically synthesized on extracellular virions. The structures displayed endogeneous polymerase activity, DNA product of the reaction being predominantly associated with 60S RNA. No 70S RNA was found in the cell structures of various densities. Also, the virions purified from tissue culture fluid contained 70S RNA. These findings are consistent with those on extracellular maturation of oncornavirus RNA.
机译:从底特律-6和人羊膜细胞的细胞质中分离了两种病毒特异性结构,以及产生oncornavirus样颗粒的人胚胎细胞。这些结构表示直径为70至80nm的颗粒,并且具有直径为3和6nm的核衣壳的聚集线。通过线性蔗糖梯度的样品植物系骨,并随后通过沉降在速度蔗糖梯度中进一步纯化该结构。它们的沉降系数分别为250和150 s 。这两个结构含有60,45和35℃ RNA物种,150℃和150℃也含有20 S RNA。来自150 S 结构的35和20 S RNA形成了杂交物,其具有酶促合成的DNA在细胞外病毒中。结构显示了内雄聚合酶活性,反应的DNA产物主要与60 S RNA相关。在各种密度的细胞结构中发现了NO 70 S RNA。此外,从组织培养流体纯化的病毒粒子含有70 S RNA。这些发现与那些on Cornavirus RNA细胞外成熟的结果一致。

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