• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of Virology >Antigenic Phenotypes and Complementation Groups of Temperature-Sensitive Mutants of Simian Virus 40
【24h】

Antigenic Phenotypes and Complementation Groups of Temperature-Sensitive Mutants of Simian Virus 40

机译：猿猴病毒40的抗原表型和互补突变体的互补组

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The antigenic phenotypes of several temperature-sensitive mutants of simian virus 40 were determined by an immunofluorescence microtechnique that allowed a very high degree of internal control for the conditions of virus infection and antigenic staining. The tumor (T), U, capsid protein (C), and virion (V) antigens were investigated. Productive infection in monkey cells and abortive infection in mouse cells were simultaneously monitored for antigen production at both permissive and restrictive temperatures. Complementation analyses of the mutants demonstrated two complementing groups (A and B) and one noncomplementing group (*). One of the complementing groups could be subdivided into two subgroups having very different antigenic phenotypes. The following phenotypes were observed at the restrictive temperature in monkey cells. (i) The noncomplementing group produced none of the antigens. (ii) Group A induced T antigen in moderately but consistently reduced numbers of cells. Other antigens were markedly reduced or absent. (iii) Some of the group B mutants produced T antigen but little or no U and V antigens. The C antigen appeared in the nucleolus and cytoplasm of this subgroup. (iv) In the other group B mutants, antigen synthesis was not altered. Similar phenotypes were observed in mouse cells, except that U, C, and V antigens could not be detected during either the mutant or wild-type virus infections at any temperature.
机译：Simian病毒40的若干温度敏感突变体的抗原表型由免疫荧光微调来确定，允许对病毒感染和抗原染色的条件进行非常高度的内部控制。研究了肿瘤（T），U，衣壳蛋白（C）和病毒酮（V）抗原。在允许和限制温度下，同时监测猴细胞中的生产感染并在小鼠细胞中进行中止感染进行抗原产生。突变体的互补分析证明了两个补充基团（A和B）和一个不合解基团（ * ）。可以将其中一种补充基团细分为两个具有非常不同的抗原表型的亚组。在猴细胞的限制温度下观察到以下表型。 （i）非符合群体不产生任何抗原。 （ii）在适度但始终减少多数细胞中诱导T抗原。其他抗原明显减少或不存在。 （iii）B组突变体的一些产生T抗原，但很少或没有U和V抗原。 C抗原出现在该亚组的核仁和细胞质中。 （iv）在另一组B突变体中，抗原合成没有改变。在小鼠细胞中观察到类似的表型，除了在任何温度的突变体或野生型病毒感染期间无法检测到U，C和V抗原。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号