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Structural Aberrations in T-Even Bacteriophage IV. Parameters of Induction and Formation of Lollipops

机译:T-均匀噬菌体IV中的结构像差。戒液的诱导和形成参数

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Previous results from our laboratory have shown that when a T-even bacteriophage-infected bacterial cell was exposed to l-canavanine followed by an l-arginine chase, a monster phage particle, termed a lollipop, was formed. We now describe certain parameters concerning (i) the induction and (ii) the formation of T4 lollipops. The induction step involves a T4 late function, and can require only a 3-min exposure to l-canavanine. Short pulses of l-canavanine result in the formation of shorter lollipops indicating the presence of a possible “precursor substance” which is influenced by l-canavanine. DNA synthesis is inhibited by l-canavanine but is stimulated 20 to 40 min after the addition of l-arginine. Chloramphenicol prevents both responses indicating a possible protein involvement. The appearance of lollipops and phage was noted only after 25 min after the addition of l-arginine.
机译:我们实验室的先前结果表明,当将T型均匀的细菌细菌细胞暴露于L-胰岛素后,然后是L-精氨酸序列,形成怪物噬菌体颗粒,称为棒棒糖。我们现在描述了某些参数关于(i)诱导和(ii)T4棒棒糖的形成。诱导步骤涉及T4晚期功能,并且可以仅需要3分钟的暴露于L- Canavanine。 L-Canavanine的短脉冲导致较短的棒棒糖,表明可能的“前体物质”存在,其受L-甘蔗脒的影响。通过L-甘蔗脒抑制DNA合成,但在加入L-精氨酸后刺激20至40分钟。氯霉素可防止两个反应表明可能的蛋白质受累。在添加L-精氨酸后25分钟后,才注意棒棒糖和噬菌体的外观。

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