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首页> 外文期刊>Journal of Virology >Effects of Actinomycin D on the Cytopathology Induced by Poliovirus in HEp-2 Cells
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Effects of Actinomycin D on the Cytopathology Induced by Poliovirus in HEp-2 Cells

机译：放线霉素D对脊髓灰质炎症患者脊髓灰质炎细胞诱导的缩细胞病变的影响

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摘要

One possible mechanism of virus-induced cell damage is that the redistributed (released) lysosomal enzymes produce the cytopathic effect during cytolytic types of infections such as poliovirus in HEp-2 cells. To determine if the lysosomal enzyme redistribution and cell damage are host-cell directed, we studied sensitivity of these events to the action of actinomycin D. By the use of actinomycin D at concentrations producing the least toxicity but maximal effectiveness in shuting down cell RNA synthesis, it was shown that the cytopathic effect and enzyme redistribution were not inhibited and, therefore, not directly controlled and induced by the cell genome in response to the virus infection. Evaluation of cytopathic effect by a phase contrast microscopy method detected changes earlier than the erythrocin B uptake method.
机译：病毒诱导的细胞损伤的一种可能机制是重新分配（释放的）溶酶体酶在细胞溶解类型的感染期间产生细胞病变效果，例如恒温血管病毒在HEP-2细胞中。为了确定溶酶体酶再分配和细胞损伤是针对宿主细胞的指导，我们研究了这些事件对放线霉素D的作用的敏感性。通过在产生最小毒性的浓度下使用放线霉素D，而是在关闭细胞RNA合成中的最大效果，结果表明，不抑制细胞病变效果和酶再分分配，因此不受细胞基因组直接控制和诱导的病毒感染。通过相位对比显微镜方法评估细胞病变效果检测到比红细胞B吸收法更换的变化。

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