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首页> 外文期刊>Journal of Virology >Cold-Sensitive Mutants of Bacteriophage ΦX174. II. Comparison of Two Cold-Sensitive Mutants
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Cold-Sensitive Mutants of Bacteriophage ΦX174. II. Comparison of Two Cold-Sensitive Mutants

机译：噬菌体φX174的冷敏感突变体。 II。两种冷敏感突变体的比较

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Cold-sensitive bacteriophage ΦX174 mutants, another class of conditional lethals, were examined with regard to growth parameters, DNA synthesis, and particle properties. Two mutants, cs70 and cs82, were examined. Mutant cs70 was eclipse defective, showing altered eclipse kinetics at permissive temperature (40 C) and failing entirely to eclipse at restrictive temperature (25 C). Mutant cs70 replicated well at 25 C if allowed prior eclipse at 40 C. Mutant cs82 had wild-type eclipse at both temperatures but was defective in single-strand synthesis at 25 C, which led to delayed progeny phage appearance, decreased progeny phage synthesis rate, and greatly reduced burst size. The cs82 block could not be bypassed by temperature shift. Since complementation analysis of cs70 and cs82 was not feasible due to the unique properties of these mutants, those ΦX174 properties affected by the virus coat were examined as an index of a mutation in a coat protein gene. Mutant cs70 had aberrant attachment kinetics at both 25 C and 40 C, evidence of a coat protein alteration. Mutant cs70 also exhibited significantly decreased thermal stability, further evidence of an altered virus structure. Mutant cs82 had increased thermal stability, but the difference was not sufficient to allow unequivocal assignment of this mutant to a coat protein gene. Both mutants had wild-type antiserum inactivation and host range, although cs70 was subject to less of (low-level) plating restriction by endogenous F+ factors.
机译：在生长参数，DNA合成和颗粒性质方面，检查冷敏感的噬菌体φX174突变体。检查了两个突变体， Cs 70和 CS 82。突变体 cs 70是Eclipse有缺陷，显示允许温度（40℃）的改变的Eclipse动力学，并完全在限制温度（25℃）处的Eclipse。如果允许在40℃的Eclipse允许在40℃的突变体 CS 82处均为突变体 Cs 70在两个温度下具有野生型Eclipse但在单链合成中有缺陷25℃，导致后代噬菌体外观延迟，后代噬菌体合成率降低，大大降低突破大小。通过温度偏移无法绕过 CS 82块。由于由于这些突变体的独特性质，因此由于这些突变体的独特性，因此对受病毒涂层影响的那些φ174性质的互补分析是不可行的。在外套蛋白基因中的突变。突变体 Cs 70在25c和40℃下具有异常的附着动力学，证据表明蛋白质改变。突变体 cs 70也表现出显着降低的热稳定性，进一步证据了病毒结构的进一步证据。突变体 cs 82具有增加的热稳定性，但差异不足以使该突变体的不确定将该突变物分配给涂层蛋白基因。两个突变体具有野生型抗血清灭活和宿主范围，但是通过内源性F + 因子受到较少（低级）电镀限制的影响。

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