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首页> 外文期刊>Journal of Virology >Biochemistry of DNA-Defective Amber Mutants of Bacteriophage T4 IV. DNA Synthesis in Plasmolyzed Cells
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Biochemistry of DNA-Defective Amber Mutants of Bacteriophage T4 IV. DNA Synthesis in Plasmolyzed Cells

机译：噬菌体T4 IV的DNA缺陷琥珀色突变体的生物化学。 DNA在等离子体分解细胞中的合成

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Requirements for bacteriophage T4 DNA synthesis have been investigated in situ by use of plasmolyzed infected cells. When such cells are incubated with dATP, dGTP, dTTP, hydroxymethyldeoxycytidine triphosphate, and rATP, significant semiconservative synthesis of DNA occurs. This DNA hybridizes preferentially to T4 DNA. T4 amber mutants defective in genes 44 and 45, which display a DNA-negative phenotype in vivo, are unable to synthesize DNA in situ. By contrast, T4 amber mutants bearing lesions in genes 41 and 62, which also display a DNA-negative phenotype in vivo, do allow DNA synthesis in situ, the extent of synthesis being 80 to 90% that of the wild-type synthesis under the same conditions. Cells infected with gene 42 mutants (dCMP hydroxymethylase) are unable to synthesize DNA in situ even though exogenous nucleotides are provided. Also one gene 1 mutant (deoxynucleotide kinase) was found to synthesize DNA in situ, but two other gene 1 mutants did not. These results point to possible roles of hydroxymethylase and kinase in DNA metabolism, in addition to provision of essential DNA precursors, as has recently been suggested by Wovcha et al. (1973).
机译：通过使用等离子体分解的细胞原位研究了噬菌体T4 DNA合成的要求。当将这种细胞与DATP，DGTP，DTTP，羟甲基二氧胞嘧啶胞嘧啶三磷酸盐和RATP孵育时，发生显着的半透明合成DNA。该DNA优先与T4 DNA杂交。在体内显示DNA阴性表型的基因44和45中有缺陷的T4琥珀色突变体无法以原位合成DNA。相比之下，T4琥珀突变体在基因41和62中轴承病变，其在体内显示DNA阴性表型，确实允许DNA合成原位，合成程度为80％至90％的野生型合成相同的条件。即使提供了外源核苷酸，用基因42突变体（DCMP羟甲基甲基酶）感染的细胞也无法以原位合成DNA。还发现一种基因1突变体（脱氧核苷酸激酶）以原位合成DNA，但另外两种基因1突变体没有。除了提供必需的DNA前体之外，这些结果表明羟甲基甲基酶和激酶在DNA代谢中的可能作用，除了WovCha等人的建议。 （1973）。

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