Newcastle disease virus-specific RNA: poly(A)-containing and poly(A)-deficient transcripts as revealed by chromatography on poly(U)-sepharose.

N L Varich; I S Lukashevich; N V Kaverin

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Newcastle disease virus-specific RNA: poly(A)-containing and poly(A)-deficient transcripts as revealed by chromatography on poly(U)-sepharose.

机译：新宫疾病病毒特异性RNA：聚（a） - 聚（a） - 聚（a） - 聚（U）色谱透露的缺点转录物 - 溶血性。

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Total [3H]uridine-labeled, virus-specific RNA from Newcastle disease virus-infected cells was fractionated by poly(U)-sepharose chromatography and analyzed by rate zonal gradient centrifugation. The sedimentation pattern of both eluted and nonadsorbed RNA resembled that of the total RNA. However, nonadsorbed RNA was enriched in 50S material, ant its 18S peak was broader and slightly shifted towards the top of the gradient. Poly(U-sepharose chromatography of isolated 18S RNA and 24S RNA resulted in the separation of poly(A)-containing RNA and poly(A)-deficient RNA. In the former the percentage of adenosine content represented by poly(A) sequences was estimated as 10 to 12% (for 18S RNA) or approximately 6.0% (for 24S RNA). The size of poly(A) fragments as measured by their sedimentation rate was the same for 18S and 24S RNA. Polyacrylamide gel electrophoresis of poly(A)-containing RNA revealed a characteristic pattern closely resembling the pattern of nonchromatographed 18S and 24S RNA. The pattern of poly(A)-deficient RNA was heterogenous, and for 18S RNA it shifted towards the anode. The possible origin of poly(A)-deficient transcripts is discussed.

机译：尿苷标记的尿苷标记，来自新城疫病毒感染细胞的尿素特异性RNA通过聚（U） - 次膦素色谱法分离，并通过速率区梯度离心分析。两种洗脱和非吸附RNA的沉降模式类似于总RNA的沉降模式。然而，在50s材料中富集非吸附的RNA，蚂蚁其18s峰越宽，略微偏移到梯度的顶部。聚（u-Sepharose的分离的18S RNA和24秒RNA导致聚（a） - 统计的RNA和聚（a） - 缺料RNA分离。在前者中，聚（a）序列表示的腺苷含量的百分比估计为10至12％（18℃）或约6.0％（24秒）。通过它们的沉降速率测量的聚（a）片段的尺寸对于18秒和24s的RNA相同。聚丙烯酰胺凝胶电泳的聚丙烯酰胺凝胶电泳（ a）揭示RNA揭示了一种与非十四种分离的18秒和24s RNA的图案密切相关的特征图案。聚（a） - 缺少RNA的图案是异源的，并且它朝向阳极偏移18秒的RNA。聚（a讨论了缺陷转录物。

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《Journal of Virology》 |1976年第1期|共6页
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N L Varich; I S Lukashevich; N V Kaverin;
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收录信息美国《科学引文索引》(SCI);
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1. Newcastle disease virus-specific RNA: poly(A)-containing and poly(A)-deficient transcripts as revealed by chromatography on poly(U)-sepharose. [J] . N L Varich, I S Lukashevich, N V Kaverin Journal of Virology . 1976,第1期

机译：新宫疾病病毒特异性RNA：聚（a） - 聚（a） - 聚（a） - 聚（U）色谱透露的缺点转录物 - 溶血性。
2. Newcastle disease virus-specific RNA: poly(A)-containing and poly(A)-deficient transcripts as revealed by chromatography on poly(U)-sepharose. [J] . N L Varich, I S Lukashevich, N V Kaverin Journal of Virology . 1976,第1期

机译：新宫疾病病毒特异性RNA：聚（a） - 聚（a） - 聚（a） - 聚（U）色谱透露的缺点转录物 - 溶血性。
3. Systematic Profiling of Poly(A)+ Transcripts Modulated by Core 3’ End Processing and Splicing Factors Reveals Regulatory Rules of Alternative Cleavage and Polyadenylation [J] . Wencheng Li, Bei You, Mainul Hoque, PLoS Genetics . 2015,第4期

机译：核心3'末端加工和剪接因子调节的Poly（A）+转录本的系统分析揭示了选择性切割和聚腺苷酸化的调控规则。
4. Langmuir and Langmuir-Blodgett-kuhn Films of Poly(vinylidene fluoride) and Poly(vinylidene fluoride-co-trifluoroethylene) Alternated with Poly(methyl methacrylate) or Poly(octadecyl methacrylate) [C] . Rigobarto C. Advincula, Wolfgang Knoll, Lev Blinov, National meeting of the American Chemical Society . 1997

机译：聚（偏二氟乙烯）和聚（偏二氟乙烯-三氟乙烯共聚物）与聚（甲基丙烯酸甲酯）或聚（甲基丙烯酸十八烷基酯）交替的Langmuir和Langmuir-Blodgett-kuhn膜
5. Retention behavior of poly(methyl methacrylate), poly(ethylene glycol) and poly(tryptophan) in reversed-phase liquid chromatography. [D] . Jiang, Chun. 1994

机译：聚（甲基丙烯酸甲酯），聚（乙二醇）和聚（色氨酸）在反相液相色谱中的保留行为。
6. Newcastle disease virus-specific RNA: poly(A)-containing and poly(A)-deficient transcripts as revealed by chromatography on poly(U)-sepharose. [O] . N L Varich, I S Lukashevich, N V Kaverin 1976

机译：新城疫病毒特异的RNA：含有poly（A）和poly（A）缺陷的转录本如在poly（U）-sepharose上进行色谱分析所揭示。
7. Systematic profiling of poly(A)+ transcripts modulated by core 3' end processing and splicing factors reveals regulatory rules of alternative cleavage and polyadenylation. [O] . Wencheng Li, Bei You, Mainul Hoque, 2015

机译：通过核心3'末端加工和剪接因子调节的poly（a）+转录物的系统分析揭示了备选切割和多腺苷酸化的调节规则。
8. Acute and Repeated Administration of Poly Inosinic - Poly Cytidylic Acid (Poly I-C), Poly Inosinic Acid (Poly I), and Poly Cytidylic (Poly C) in Beagle Dogs. Volume I [R] . Zendzian, R. P., Teeters, W. R., Homan, E. R., 1970

机译：在比格犬中急性和重复给予多肌苷 - 聚胞苷酸（聚I-C），聚肌苷酸（聚I）和聚胞苷（聚C）。第一卷

Newcastle disease virus-specific RNA: poly(A)-containing and poly(A)-deficient transcripts as revealed by chromatography on poly(U)-sepharose.

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