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首页> 外文期刊>Journal of Virology >Function of gene 49 of bacteriophage T4. II. Analysis of intracellular development and the structure of very fast-sedimenting DNA.
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Function of gene 49 of bacteriophage T4. II. Analysis of intracellular development and the structure of very fast-sedimenting DNA.

机译：噬菌体T4基因49的功能。 II。细胞内发育分析及非常快速沉积DNA的结构。

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摘要

With the exception of mutants in gene 49, all mutants in phage T4 defective in the process of head filling accumulate a normal replicative DNA intermediate of 200S. Mutants in gene 49 produce a very fast-sedimenting (VFS) DNA with s values of greater than 1,000S. The intracellular development of the VFS-DNA generated in gene 49-defective phage-infected cells was followed by sedimentation analysis of crude lysates on neutral sucrose gradients. It was observed that the production of a 200S replicative intermediate is one step in the development of VFS-DNA. After restoring permissive conditions the development of the VFS-DNA can be reversed, but the 200S form is not regenerated under these conditions. The process of head filling can take place from the VFS-DNA under permissive conditions. From the absence of other components in the VFS-DNA complexes, its high resistance to shearing, its resistance against the attack of the single-strand-specific nuclease S1, and from its appearance in the electron microscope, a complex structure of tightly packed DNA is inferred. The demonstration by the electron microscope of branched DNA structures sometimes closely related to partially filled heads is taken in support of the idea that the process of head filling in gene 49-defective phage-infected cells is blocked by some steric hindrance in the DNA. In light of these results, the role of gene 49 is discussed as a control function for the clearance of these structures. A fixation procedure for cross-linking of gene 49-defective heads to the VFS-DNA allowed us to study progressive stages in the process of head filling. Electron microscopic evidence is presented which suggests that during the initial events the DNA accumulates in the vertexes of the head.
机译：除了基因49中的突变体外，噬菌体T4中的所有突变体在头填充过程中缺陷，积累了200多岁的正常复制DNA中间体。基因49中的突变体产生非常快的沉积（VFS）DNA，其值大于1000s。在基因49型缺陷噬菌体感染细胞中产生的VFS-DNA的细胞内发育随后在中性蔗糖梯度上的粗裂解物沉降分析。观察到，200多岁的复制中间体的产生是VFS-DNA的发育的一步。在恢复允许条件后，VFS-DNA的开发可以逆转，但在这些条件下，200S的形式不会再生。头枕的过程可以在允许条件下从VFS-DNA发生。从VFS-DNA复合物中没有其他组分，其高抗剪切，其抗于单链特异性核酸酶S1的抗侵蚀，并且从其在电子显微镜中的外观，紧密填充DNA的复杂结构推断出来。用于支化DNA结构的电子显微镜的演示有时与部分填充头部密切相关，以支持基因49缺陷的噬菌体感染细胞中的头部填充过程中的一些空间阻断。鉴于这些结果，基因49的作用被讨论为用于这些结构的间隙的控制功能。用于对VFS-DNA的基因49缺陷头的交联的固定程序使我们能够研究头部填充过程中的渐进阶段。提出了电子显微验证，这表明在初始事件期间DNA在头部的顶点中积聚。

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    《Journal of Virology》 |1976年第3期|共16页
  • 作者

    B Kemper; D T Brown;

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  • 收录信息 美国《科学引文索引》(SCI);
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