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首页> 外文期刊>Journal of Virology >Characterization of an RNA-dependent RNA polymerase activity associated with measles virus.
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Characterization of an RNA-dependent RNA polymerase activity associated with measles virus.

机译：表征与麻疹病毒相关的RNA依赖性RNA聚合酶活性。

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摘要

An RNA-dependent RNA polymerase activity has been found copurifying with measles virus infectivity and complement-fixing antigen in three Vero cell-grown variants of measles virus: the attenuated Edmonston B strain, the natural non-attenuated Edmonston strain, and a subacute sclerosing panencephalitis isolate, IP-3. Incubation of purified measles virions with immunoglobulin G derived from sera of monkeys hyperimmunized against measles specifically removes activity sedimenting in the density region of measles virions. The requirements of the reaction, which is RNase sensitive, are similar to those reported for other paramyxovirus-associated activities, including detergent, divalent cation, ribonucleoside triphosphates, and a reducing agent. The size classes of RNA synthesized correspond to those found in measles-infected cells, including 50, 35, and 16 to 20S. The product RNA of the Edmonston B virus-stimulated reaction was rendered RNase resistant by annealing with RNA extracted from purified Edmonston B virions. RNA from uninfected Vero cells was ineffective in the annealing reaction.
机译：已发现RNA依赖性RNA聚合酶活性与麻疹病毒的麻疹病毒感染性和补体固定抗原共饱和：减毒的Edmonston B株，天然未减毒的edmonston菌株和亚急性硬化终连脑炎孤立，IP-3。纯化麻疹病毒素与免疫球蛋白G衍生自猴子的血清血清，超中可抵抗麻疹的密度清除在麻疹病毒粒子的密度区域中的活性沉淀。作为RNase敏感的反应的要求与其他副缺陷病毒相关活性的反应类似，包括洗涤剂，二价阳离子，核糖核苷三磷酸和还原剂。合成的RNA的大小等级对应于在麻疹感染细胞中发现的那些，其中包括50,35和16至20s。通过用从纯化的edmonstonb病毒粒子提取的RNA退火，通过退火使edmonstonb病毒刺激反应的产物RNA进行了抗性。来自未感染的Vero细胞的RNA在退火反应中是无效的。

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