• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of Virology >Vesicular stomatitis virus mutants resistant to defective-interfering particles accumulate stable 5'-terminal and fewer 3'-terminal mutations in a stepwise manner.
【24h】

Vesicular stomatitis virus mutants resistant to defective-interfering particles accumulate stable 5'-terminal and fewer 3'-terminal mutations in a stepwise manner.

机译：耐缺陷干扰颗粒的囊泡口炎病毒突变体逐步地积聚稳定的5'-末端和更少的3'-末端突变。

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

We have studied the evolution of sequences which include the RNA polymerase binding sites at the 5' and 3' termini of vesicular stomatitis virus mutants (Sdi-) resistant to defective-interfering particles. We observed a striking stepwise accumulation of stable base substitutions within the area of replication initiation at the 5'-terminal 54 nucleotides of Sdi- mutants isolated at intervals from persistent infections and undiluted lytic passage series. Fewer mutations accumulated in the region of transcription initiation at the 3' end and in those portions of the N and L protein coding cistrons examined. The termini changes are not strictly required to obtain the Sdi- phenotype. However, it is possible that they represent stepwise compensatory changes to accommodate Sdi- mutations affecting viral replication or encapsidation gene products or both. These results have important implications for RNA virus genome evolution.
机译：我们已经研究了序列的演变，其包括在5'和3'末端的RNA聚合酶结合位点，其抗体缺陷干扰颗粒耐缺陷的颗粒晶状体突变体（SDI-）。我们观察到在5'-突变体54核苷酸的复制引发区域内逐步积聚在持续感染的间隔和未稀释的裂变通道系列的间隔分离的5'-突变体54核苷酸的核苷酸内。在3'末端的转录起始区域中累积的突变较少，并且在据检查的N和L蛋白质编码传感器的那些部分中。末端的变化是不严格要求获得SDI-表型。然而，它们可以代表逐步补偿变化以适应影响病毒复制或封装基因产物或两者的SDI-突变。这些结果对RNA病毒基因组进化具有重要意义。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号