• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of Virology >Fv-1 host cell restriction of friend leukemia virus: microinjection of unintegrated viral DNA.
【24h】

Fv-1 host cell restriction of friend leukemia virus: microinjection of unintegrated viral DNA.

机译：FV-1宿主细胞限制朋友白血病病毒：未受整治病毒DNA的显微注射。

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The murine gene Fv-1 has been shown to exert a major influence over the replication of ecotropic murine leukemia viruses. Studies of the replication of Friend murine leukemia virus have shown that the restriction of viral replication occurs intracellularly after the initiation of viral DNA synthesis. The precise mechanism of the block imposed by the Fv-1 gene product is not completely understood. Our studies of Fv-1 restrictive infection have shown a variable decrease in the accumulation of intracellular unintegrated form I viral DNA. Analysis by microinjection of the viral DNA formed in nonpermissively infected BALB/c cells indicates that this DNA is infectious. These studies indicate that the form I DNA accumulated in nonpermissively infected BALB/c cells contains the complete viral sequences necessary for the production of viral progeny, and therefore, they suggest that the Fv-1 host restrictive mechanism recognizes viral factors other than form I DNA alone. These results support the possibility that Fv-1 host restriction occurs after formation of infectious viral DNA, perhaps at the integration step itself.
机译：鼠基因FV-1已被证明对生态鼠白血病病毒的复制产生了重大影响。对朋友鼠白血病病毒复制的研究表明，在引发病毒DNA合成后，细胞内发生病毒复制的限制。不完全理解由FV-1基因产物施加的块的精确机制。我们对Fv-1限制性感染的研究表明，细胞内未分解形式的病毒DNA的积累变化降低。通过在非智能感染的BALB / C细胞中形成的病毒DNA的显微注射分析表明该DNA是感染性的。这些研究表明，在非智能感染的BALB / C细胞中累积的I DNA的形式含有生物后代生产所需的完整病毒序列，因此，他们表明FV-1宿主限制机制识别除I形式外的病毒因子独自的。这些结果支持在形成传染性病毒DNA之后发生Fv-1宿主限制的可能性，也许在积分步骤本身。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号