Direct demonstration that the abundant 6-kilobase herpes simplex virus type 1 mRNA mapping between 0.23 and 0.27 map units encodes the major capsid protein VP5.

R H Costa; G Cohen; R Eisenberg; D Long; E Wagner

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Direct demonstration that the abundant 6-kilobase herpes simplex virus type 1 mRNA mapping between 0.23 and 0.27 map units encodes the major capsid protein VP5.

机译：直接证明，其丰富的6千碱基疱疹单纯乳剂型1 mRNA映射在0.23和0.27的地图单元之间进行编码主要衣壳蛋白VP5。

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The two partially colinear 6-kilobase (kb) and 1.5-kb mRNAs mapping between 0.23 and 0.27 map units on the herpes simplex virus type 1 genome were precisely located. The 5' end of the 6-kb mRNA was located 28 bases downstream of the sequence ATATATT and was 10 bases to the left of the BamHI site at 0.268. This position is ca. 90 bases to the left of our earlier reported sequence (R. J. Frink, K. G. Draper, and E. K. Wagner, Proc. Natl. Acad. Sci. U.S.A. 78:6139-6143, 1981). We used a polyclonal antibody made against purified herpes simplex virus type 1 VP5 to demonstrate that the 155,000-dalton translation product of the 6-kb mRNA is this capsid protein. The antibody did not react with the 35,000-dalton translation product of the 1.5-kb mRNA. We also confirmed our identification of VP5 as the translation product of the 6-kb mRNA by comparison of tryptic peptides of the in vitro-translated protein and authentic VP5.

机译：在疱疹病毒型1基因组上的0.23和0.27映射单位之间的两个部分的半阴影6千碱（Kb）和1.5 kb mRNA映射。 6-KB mRNA的5'末端位于序列atatatt下游的28个碱基，并且在0.268的BamHI位点的左侧是10个碱基。这个位置是加利福尼亚州。 90个碱基向我们之前的报告序列的左侧（R.J. Frink，K.G. Draper和E. K.Wagner，Proc。Natl。Acad。SCI。U.S.A. 78：6139-6143,1981）。我们使用针对纯化疱疹病毒的多克隆抗体1 VP5，证明了6-KB mRNA的155,000-Dalton翻译产物是该胶囊蛋白。抗体与1.5 kB mRNA的35,000-dalton翻译产物没有反应。我们还通过对体外翻译的蛋白质和真正的VP5的胰蛋白酶肽进行了比较，证实我们的鉴定为6-KB mRNA的翻译产品。

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《Journal of Virology》 |1984年第1期|共6页
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R H Costa; G Cohen; R Eisenberg; D Long; E Wagner;
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1. Direct demonstration that the abundant 6-kilobase herpes simplex virus type 1 mRNA mapping between 0.23 and 0.27 map units encodes the major capsid protein VP5. [J] . R H Costa, G Cohen, R Eisenberg, Journal of Virology . 1984,第1期

机译：直接证明，其丰富的6千碱基疱疹单纯乳剂型1 mRNA映射在0.23和0.27的地图单元之间进行编码主要衣壳蛋白VP5。
2. Direct demonstration that the abundant 6-kilobase herpes simplex virus type 1 mRNA mapping between 0.23 and 0.27 map units encodes the major capsid protein VP5. [J] . R H Costa, G Cohen, R Eisenberg, Journal of Virology . 1984,第1期

机译：直接证明，其丰富的6千碱基疱疹单纯乳剂型1 mRNA映射在0.23和0.27的地图单元之间进行编码主要衣壳蛋白VP5。
3. Second-site mutations encoding residues 34 and 78 of the major capsid protein (VP5) of herpes simplex virus type 1 are important for overcoming a blocked maturation cleavage site of the capsid scaffold proteins [J] . Warner SC., Person S., Desai P. Virology . 2000,第1期

机译：编码1型单纯疱疹病毒主要衣壳蛋白（VP5）的34和78位残基的第二位突变对于克服衣壳支架蛋白的受阻成熟裂解位点很重要
4. Characterization of VP26 Capsid Protein during Herpes Simplex Virus Type 1 Assembly [C] . Daniell L. Rowles, Todd M. Greco, Aaron E. Lin, American Society for Mass Spectrometry Conference on Mass Spectrometry and Allied Topics . 2011

机译：疱疹病毒型1组装疱疹病毒中VP26辣椒蛋白的表征
5. Insights into the interaction and nuclear capsid-association of herpes simplex virus type 1 tegument proteins UL36 and UL37. [D] . Bucks, Michelle Ann. 2010

机译：深入了解单纯疱疹病毒1型外皮蛋白UL36和UL37的相互作用和核衣壳缔合。
6. Direct demonstration that the abundant 6-kilobase herpes simplex virus type 1 mRNA mapping between 0.23 and 0.27 map units encodes the major capsid protein VP5. [O] . R H Costa, G Cohen, R Eisenberg, 1984

机译：直接证明在0.23和0.27映射单元之间映射的丰富的6碱基的单纯疱疹病毒1型mRNA编码主要衣壳蛋白VP5。
7. Second-Site Mutations Encoding Residues 34 and 78 of the Major Capsid Protein (VP5) of Herpes Simplex Virus Type 1 Are Important for Overcoming a Blocked Maturation Cleavage Site of the Capsid Scaffold Proteins [O] . Warner Susan C., Desai Prashant, Person Stanley 2000

机译：编码1型单纯疱疹病毒主要衣壳蛋白（VP5）残基的34-78位第二位突变对于克服衣壳支架蛋白的受阻成熟切割位点很重要
8. Identification and Characterization of the UL37 Protein of Herpes Simplex Virus Type 1 and Demonstration that it Interacts with ICP8, the Major DNA Binding Protein of Herpes Simplex Virus. [R] . Shelton, L. 1992

机译：单纯疱疹病毒1型UL37蛋白的鉴定和鉴定及其与单纯疱疹病毒主要DNa结合蛋白ICp8的相互作用的证明。

Direct demonstration that the abundant 6-kilobase herpes simplex virus type 1 mRNA mapping between 0.23 and 0.27 map units encodes the major capsid protein VP5.

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