Identification of oligonucleotide fragments produced in a strand scisslon reaction of the d(C-G-C-G-C-G) duplex by bleomycin

Morio Ikehara; Seiichi Uesugi; Toshio Shida; Yoshimasa Kyogoku; Yuji Kobayashi

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Identification of oligonucleotide fragments produced in a strand scisslon reaction of the d(C-G-C-G-C-G) duplex by bleomycin

机译：鉴定D（C-G-C-G-C-G）双链体的股线瘢痕反应中产生的寡核苷酸片段

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To elucidate the mechanism of DNA strand scission by bleomycin, a d(C-G C-G-C-G) duplex was treated with the bleomycin-iron ion complex in the presence of H₂ O₂ and degradation products (1, 2, cytosine and deoxyguanosine 5′-phosphate were identified. 1 and 2 contain a carboxyinethyl group attached to the 3′-terminal phosphoryl group of d(C_?Gp) and₃₁ d(C-G-C-Gp), respectively. These compounds were identified by UV, H and P NMR spectroscopy and paper electrophoresis. 1 was synthesized from the protected dinucleotide and glycolic acid and the proton NMR spectrum was identical to that of 1 obtained as a degradation product. Thus the oligonucleotide fragments produced by the action of bleomycin on DNA were directly identified and cleavage of the C3′-C4′ bond of the sugar residues was proved.

机译：为了阐明通过Bleomycin的DNA链进行的机制，在H _{2 / sup> O _{2 和降解存在下，用玻璃霉素 - 铁离子复合物处理Ad（CG CGCG）双链体鉴定产物（1,2，胞嘧啶和脱氧胍5'-磷酸酯。1和2含有附着在D（C _{β gp）和_{的3'-末端磷酰基的羧基乙基。分别通过UV，H和P NMR光谱和纸电泳鉴定出这些化合物。从受保护的二核苷酸和乙醇酸合成1，质子NMR谱与其中相同1作为降解产物获得。因此，通过博莱霉素作用产生的寡核苷酸片段直接鉴定出糖霉素的脱落，并证明了糖残基的C3'-C4'键。}}}}

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《Nucleic acids research》 |1984年第3期|共12页
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Morio Ikehara; Seiichi Uesugi; Toshio Shida; Yoshimasa Kyogoku; Yuji Kobayashi;
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1. Identification of oligonucleotide fragments produced in a strand scisslon reaction of the d(C-G-C-G-C-G) duplex by bleomycin [J] . Morio Ikehara, Seiichi Uesugi, Toshio Shida, Nucleic acids research . 1984,第3期

机译：鉴定D（C-G-C-G-C-G）双链体的股线瘢痕反应中产生的寡核苷酸片段
2. Identification of oligonucleotide fragments produced in a strand scisslon reaction of the d(C-G-C-G-C-G) duplex by bleomycin [J] . Morio Ikehara, Seiichi Uesugi, Toshio Shida, Nucleic acids research . 1984,第3期

机译：鉴定D（C-G-C-G-C-G）双链体的股线瘢痕反应中产生的寡核苷酸片段
3. Sequence-specific double-strand cleavage of DNA by Fe-bleomycin. 1. The detection of sequence-specific double-strand breaks using hairpin oligonucleotides. [J] . Absalon MJ, Kozarich JW, Stubbe J Biochemistry . 1995,第6期

机译：铁博来霉素对DNA的序列特异性双链切割。 1.使用发夹寡核苷酸检测序列特异性双链断裂。
4. AN INVESTIGATION OF FRAGMENTS PRODUCED IN THE ~(58-64)Ni + ~9Be FRAGMENTATION REACTIONS [C] . Y. L. Zhang, C. W. Ma, S. S. Wang, National Conference on Nuclear Structure in China . 2016

机译：对〜（58-64）Ni +〜9BE碎片反应产生的片段的研究
5. Measurement and Modeling of Fragments and Neutrons Produced from Projectile Fragmentation Reactions [D] . Stiefel, Krystin Elizabeth 2018

机译：弹丸碎裂反应产生的碎片和中子的测量和建模
6. Identification of oligonucleotide fragments produced in a strand scission reaction of the d(C-G-C-G-C-G) duplex by bleomycin. [O] . S Uesugi, T Shida, M Ikehara, 1984

机译：鉴定由博来霉素在d（C-G-C-G-C-G）双链体的断链反应中产生的寡核苷酸片段。
7. Noncovalent intermolecular crosslinks are produced by bleomycin reaction with duplex DNA. [O] . Lloyd, R S, Haidle, C W, Robberson, D L 1979

机译：非共价分子间交联是通过博来霉素与双链体DNA反应产生的。
8. On-Line Isotope Separation of Projectile Fragments Produced in Relativistic Heavy-Ion Reactions [R] . Nojiri, Y. , Matsuta, K. , Minamisono, T. , 1986

机译：相对论重离子反应产生弹丸碎片的在线同位素分离

Identification of oligonucleotide fragments produced in a strand scisslon reaction of the d(C-G-C-G-C-G) duplex by bleomycin

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